Dissociation between intracellular calcium elevation and development of human oocytes treated with calcium ionophore

Fertility and Sterility
P RinaudoD L Keefe

Abstract

To develop an acceptable model system to study calcium activation of human oocytes. Study of oocyte development and intracellular calcium [Ca]i dynamics of activated oocytes. Research center affiliated with infertility service. Morphologic evidence of meiotic maturation and cell division under high-power Hoffman optics with an inverted microscope. Meiotic maturation was determined by the number of polar bodies or the presence of a pronucleus, and cell division was determined by evidence of a cleavage furrow or presence of blastomeres. To monitor the effect of calcium ionophore on [Ca]i levels, oocytes were incubated with fura-2 (2 microM) for 30 minutes and [Ca]i was determined by rationing the emission fluorescence (510-nm long-pass filter) during simultaneous excitation at 340 and 380 nm with a microspectrofluorimeter. All oocytes loaded with fura-2 and then exposed to ionophore exhibited an isolated elevation of [Ca]i, followed by prompt return to baseline levels. None of the oocytes showed signs of cleavage or of meiotic maturation after treatment with calcium ionophore. Human oocytes activated with calcium ionophore A23187 or ionomycin exhibited elevated [Ca]i but remained resistant to subsequent meiotic maturation and cle...Continue Reading

References

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Citations

Oct 23, 2010·Journal of Assisted Reproduction and Genetics·Laura EscrichMaría-José Escribá
Jun 13, 2008·Stem Cell Reviews·Tiziana A L BreviniFulvio Gandolfi
Sep 16, 2000·Molecular and Cellular Endocrinology·S HeyersM Montag
Jul 13, 2012·Zygote : the Biology of Gametes and Early Embryos·Karen VersierenPetra De Sutter
Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·N T RogersK Swann
Jul 31, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·K HinrichsD L Hartman
Sep 13, 2007·Stem Cell Reviews·Andrew J FrenchAlan O Trounson
Dec 21, 2013·Disability and Rehabilitation. Assistive Technology·Sue Gudgeon, Susan Kirk
Jan 26, 2016·Fertility and Sterility·María-José EscribáAntonio Pellicer
Nov 6, 2010·Reproductive Biomedicine Online·Karen VersierenPetra De Sutter
Jan 10, 2008·Cell Proliferation·T A L Brevini, F Gandolfi
Jan 11, 2014·Zygote : the Biology of Gametes and Early Embryos·Hruda Nanda MalikDhruba Malakar
May 28, 2013·European Journal of Orthopaedic Surgery & Traumatology : Orthopédie Traumatologie·May Y W WongWilliam R Walsh
Feb 18, 2020·Frontiers in Cell and Developmental Biology·Alaaeldin SalehMichail Nomikos
Dec 17, 2008·Reproductive Biomedicine Online·Elke HeytensPetra De Sutter

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