Dissociation (Ds) constructs, mapped Ds launch pads and a transiently-expressed transposase system suitable for localized insertional mutagenesis in rice

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Narayana M UpadhyayaElizabeth S Dennis

Abstract

We have developed a transiently-expressed transposase (TET)-mediated Dissociation (Ds) insertional mutagenesis system for generating stable insertion lines in rice which will allow localized mutagenesis of a chromosomal region. In this system, a Ds containing T-DNA construct was used to produce Ds launch pad lines. Callus tissues, from single-copy Ds/T-DNA lines, were then transiently infected with Agrobacterium harbouring an immobile Ac (iAc) construct, also containing a green fluorescent protein gene (sgfpS65T) as the visual marker. We have regenerated stable Ds insertion lines at a frequency of 9-13% using selection for Ds excision and GFP counter selection against iAc and nearly half of them were unique insertion lines. Double transformants (iAc/Ds) were also obtained and their progeny yielded approximately 10% stable insertion lines following excision and visual marker screening with 50% redundancy. In general, more than 50% of the Ds reinsertions were within 1 cM of the launch pad. We have produced a large number of single-copy Ds/T-DNA launch pads distributed over the rice chromosomes and have further refined the Ds/T-DNA construct to enrich for "clean" single-copy T-DNA insertions. The availability of single copy "clean...Continue Reading

References

Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Sep 1, 1995·The Plant Journal : for Cell and Molecular Biology·Y G LiuR F Whittier
May 25, 1994·Molecular & General Genetics : MGG·G N RudenkoJ Hille
Mar 1, 1996·Current Biology : CB·W ChiuJ Sheen
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·H G ChinC D Han
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·B HansonN Gutterson
Feb 5, 2000·Genome Génome / Conseil National De Recherches Canada·S TakumiC Nakamura
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·J S JeonG An
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·T KoprekP G Lemaux
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Mar 3, 2001·Current Opinion in Plant Biology·H Hirochika
Mar 13, 2001·Plant Physiology·R GrecoA Pereira
Sep 29, 2001·The Plant Journal : for Cell and Molecular Biology·S V WesleyP M Waterhouse
Feb 20, 2002·The Plant Journal : for Cell and Molecular Biology·M HaninJ Paszkowski
Apr 6, 2002·Science·Stephen A GoffSteven Briggs
Jun 5, 2002·The Plant Cell·Anthony Trewavas
Sep 10, 2002·Nature Biotechnology·Rie TeradaShigeru Iida
Jun 28, 2003·Methods : a Companion to Methods in Enzymology·Rui LuDavid C Baulcombe
Jul 19, 2003·Science·UNKNOWN Rice Full-Length cDNA ConsortiumAyako Yasunishi
Sep 17, 2003·The Plant Journal : for Cell and Molecular Biology·Shuangyan ChenPing Wu
Sep 19, 2003·Plant Molecular Biology·Sung-Ryul KimGynheung An
Nov 18, 2003·Molecular Genetics and Genomics : MGG·R GrecoA Pereira
Dec 24, 2003·The Plant Journal : for Cell and Molecular Biology·Tatiana KolesnikSrinivasan Ramachandran
Jun 29, 2004·Molecular Genetics and Genomics : MGG·Y ItoN Kurata
Jul 1, 2004·The Plant Journal : for Cell and Molecular Biology·Chul Min KimChang-deok Han
Jul 17, 2004·The Plant Journal : for Cell and Molecular Biology·Christophe SallaudEmmanuel Guiderdoni
Jul 21, 2004·Trends in Genetics : TIG·Tzvi TzfiraVitaly Citovsky
Jul 31, 2004·Plant Molecular Biology·Hirohiko HirochikaHei Leung
Jan 22, 2005·Plant & Cell Physiology·Gynheung AnSeong-Ryong Kim
Apr 22, 2005·The Plant Journal : for Cell and Molecular Biology·Smita KurupJim Haseloff
Aug 16, 2005·Nature·UNKNOWN International Rice Genome Sequencing Project
May 1, 2002·Functional Plant Biology : FPB·Narayana M UpadhyayaElizabeth S Dennis

❮ Previous
Next ❯

Citations

Mar 30, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J J Ruiz-RojasRichard E Veilleux
Nov 23, 2011·Functional & Integrative Genomics·Surinder SinghJaswinder Singh
Apr 13, 2007·Plant Molecular Biology·Michael A AyliffeAnthony J Pryor
Jul 6, 2007·Plant Molecular Biology·Sung Han ParkChang-Deok Han
Jan 8, 2009·Plant Physiology·Arjun KrishnanAndy Pereira
Mar 22, 2008·Genome Génome / Conseil National De Recherches Canada·Wei-Jiang LuanZong-Xiu Sun
Dec 18, 2013·Rice·Fu-Jin WeiYue-Ie C Hsing
Feb 3, 2016·The Plant Journal : for Cell and Molecular Biology·Fu-Jin WeiYue-Ie C Hsing
Apr 10, 2007·Trends in Plant Science·Georg Jander, Carina Barth
Aug 1, 2012·Plant Biotechnology Journal·Richard E VeilleuxVladimir Shulaev
Sep 1, 2016·Current Protocols in Plant Biology·Yuan Hu XuanChang-Deok Han
Nov 30, 2019·Frontiers in Plant Science·Vívian Ebeling VianaAntonio Costa de Oliveira

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Plant Journal : for Cell and Molecular Biology
Chellian Santhosh KumarVenkatesan Sundaresan
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
R GrecoA Pereira
© 2021 Meta ULC. All rights reserved