Dissociation of biomolecules using a ultraviolet matrix-assisted laser desorption/ionisation time-of-flight/curved field reflectron tandem mass spectrometer equipped with a differential-pumped collision cell

Rapid Communications in Mass Spectrometry : RCM
Omar BelgacemEmmanuel Raptakis

Abstract

A commercial matrix-assisted laser desorption/ionisation time-of-flight (MALDI-ToF) instrument equipped with a curved field reflectron (CFR) was modified in order to perform collision-induced dissociation (CID) on a variety of biomolecules. The incorporation of a high-resolution ion gate together with a collision cell within the field-free region allowed tandem mass analysis (MS/MS), without the necessity to decelerate the precursor ions prior to activation. The simultaneous detection of all product ions remained possible by using the CFR. To test the MS/MS performances, ACTH (fragment 1-17), a complex high mannose carbohydrate (Man)(8)(GlcNac)(2) and a lysophosphatidylcholine lipid (18:1) were analysed on the modified instrument. Direct comparison with the low-energy product ion spectra, acquired on a MALDI quadrupole ion trap (QIT) two-stage reflectron time-of flight (ReToF) mass spectrometer, showed significant differences in the types of product ions observed. The additional ions detected were a clear indication of the high-energy fragmentation processes occurring in the collision cell.

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Citations

Mar 3, 2009·Journal of the American Society for Mass Spectrometry·Ernst Pittenauer, Günter Allmaier
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Oct 16, 2008·Journal of Mass Spectrometry : JMS·Raymond March, Jennifer Brodbelt
Aug 13, 2011·Journal of Mass Spectrometry : JMS·Raymond E MarchNorman S Allen
Jul 20, 2010·Journal of Mass Spectrometry : JMS·Hirofumi NagaoMichisato Toyoda
Sep 14, 2012·Journal of Mass Spectrometry : JMS·Fabiola De MarchiRiccardo Flamini
Jan 31, 2015·Analytical and Bioanalytical Chemistry·Ernst PittenauerGünter Allmaier
Mar 24, 2016·Proteomics·Ernst PittenauerGünter Allmaier
Oct 27, 2007·Molecular & Cellular Proteomics : MCP·Wolfgang WinklerRudolf Oehler
Jan 31, 2008·Analytical Chemistry·Gerald StübigerGünter Allmaier
Mar 21, 2007·Analytical Chemistry·Gerald Stübiger, Omar Belgacem

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