Dissociation of cytochrome c from liposomes by histone H1. Comparison with basic peptides

Biochemistry
M Rytömaa, P K Kinnunen

Abstract

The basic chromosomal protein histone H1 binds avidly to liposomes containing acidic phospholipids and with characteristics somewhat resembling the lipid association of cytochrome c (cyt c) [Koiv et al. (1995) Biochemistry 34, 8018-8027]. Membrane association of histone H1 strongly attenuates the lipid lateral diffusion in large unilamellar vesicles containing phosphatidylglycerol (PG) as revealed by the decrease in the excimer to monomer ratio Ie/Im of the pyrene fatty acid-containing phospholipid derivative 1-palmitoyl-2-[10-(pyren-1-yl)decanoyl]-sn-glycero-3-phosphogly cer ol (PPDPG) fluorescence. Similarly, an increase in fluorescence anisotropy of the membrane-incorporated probe, diphenylhexatriene (DPH), due to histone H1 indicates that the membrane becomes more rigid. Increasing the mole fraction of PG (XPG) increases in a linear manner the concentration [H1]s required for the maximal decrease in Ie/Im or increase in fluorescence anisotropy, thus allowing to estimate the binding site for H1 to be constituted by approximately 20 PG molecules. Domain formation is also supported by differential scanning calorimetry measurements. Subsequently, we studied the detachment of cyt c from PG-containing liposomes by H1 by measuring...Continue Reading

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Citations

Jul 20, 2002·Biochemical and Biophysical Research Communications·Graciela A BorioliBruno Maggio
Jul 3, 2002·Chemistry and Physics of Lipids·Pentti Somerharju
May 3, 2003·Biophysical Chemistry·Galina P Gorbenko, Yegor A Domanov
Jan 15, 2000·Biochimica Et Biophysica Acta·A G Buckland, D C Wilton
Oct 29, 2002·Bioelectrochemistry·V V Kuvichkin
Apr 19, 2000·The Biochemical Journal·C StefanelliC M Caldarera
Nov 19, 2013·Biochemistry. Biokhimii︠a︡·Yu A VladimirovA V Alekseev
Jan 1, 2016·Biochemistry·Luciana HannibalRafael Radi
Jan 19, 2008·Biophysical Chemistry·Valeriya M Trusova, Galyna P Gorbenko
Nov 8, 2011·Biochemical and Biophysical Research Communications·Paolo AscenziMassimo Coletta
Feb 24, 2010·Free Radical Biology & Medicine·Giuseppe ParadiesFrancesca M Ruggiero
Apr 17, 2009·Cell Calcium·Giuseppe ParadiesFrancesca M Ruggiero
Oct 24, 2014·World Journal of Gastroenterology : WJG·Giuseppe ParadiesGiuseppe Petrosillo
Nov 7, 1998·Biochimica Et Biophysica Acta·G P Gorbenko

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