Dissociation of K channel density and ROMK mRNA in rat cortical collecting tubule during K adaptation

The American Journal of Physiology
G FrindtL G Palmer

Abstract

The density of conducting K channels in the apical membrane of the rat cortical collecting tubule (CCT) is increased by a high-K diet. To see whether this involved increased abundance of mRNA coding for K channel protein, we measured the relative amounts of mRNA for ROMK, the clone of the gene thought to encode the secretory K channel in the CCT. Tubules were isolated and fixed for in situ hybridization with a probe based on the ROMK sequence. Radiolabeled probe associated with the tubule was quantified using densitometric analysis of the autoradiographic images of the tubules. The densitometry signal was shown to be proportional to the amount of radioactive probe in the sample and to the time of exposure of the film. The technique was able to detect an approximately twofold increase in the abundance of mRNA coding for the water channel aquaporin 3 (AQP3), in response to a 30-h dehydration period. Tubules from rats fed a normal diet or a high-K (10% KCl) diet had equal amounts of ROMK mRNA. This suggests that an increase in the abundance of mRNA does not underlie the increase in channel density observed under these conditions.

References

Jan 1, 1992·Annual Review of Physiology·W WangG Giebisch
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M EchevarriaG Frindt
Mar 1, 1994·The American Journal of Physiology·H ZhouL G Palmer
Jan 1, 1997·Annual Review of Physiology·W WangG Giebisch

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Citations

Aug 13, 2010·American Journal of Physiology. Renal Physiology·Gustavo Frindt, Lawrence G Palmer
Feb 8, 2000·American Journal of Physiology. Renal Physiology·W WangG Giebisch
Dec 25, 2004·Physiological Reviews·Steven C HebertWenhui Wang
May 22, 2009·American Journal of Physiology. Renal Physiology·Paul A Welling, Kevin Ho
Jul 18, 2001·American Journal of Physiology. Renal Physiology·Y WeiW H Wang
Jun 3, 2000·American Journal of Physiology. Renal Physiology·P A MennittL G Palmer
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Wei-Zhong ZengChou-Long Huang
May 26, 2005·American Journal of Physiology. Renal Physiology·Fadi NajjarLisa M Satlin
Sep 4, 2004·American Journal of Physiology. Renal Physiology·Pierre MenetonDavid G Warnock
Nov 24, 2001·The Journal of Biological Chemistry·Hyacinth SterlingWen-Hui Wang
Sep 4, 2003·American Journal of Physiology. Renal Physiology·Po-Yin ChuChou-Long Huang
Apr 30, 2010·American Journal of Physiology. Renal Physiology·Rolando Carrisoza-GaytánLaura I Escobar
Feb 24, 2004·Annual Review of Physiology·WenHui Wang
Mar 20, 2009·American Journal of Physiology. Renal Physiology·Heidi FodstadJean-Daniel Horisberger

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