Dissociation of long and very long chain fatty acids from phospholipid bilayers

Biochemistry
F ZhangJames A Hamilton

Abstract

Dissociation of fatty acids (FA) from and transbilayer movement (flip-flop) in small unilamellar phosphatidylcholine vesicles (SUV) were monitored by measuring the pH inside the vesicle with an entrapped water-soluble fluorophore, pyranin. With a pH gradient imposed upon SUV preloaded with FA, the rate of flip-flop of saturated very long chain FA (C20:0, C:22:0, and C24:0) was shown to be fast (t1/2 < 1 s); previously, we showed by stopped flow measurements that flip-flop of long chain (14-18 carbons) FA is very fast [t1/2 < 10 ms; Kamp, F., et al. (1995) Biochemistry 34, 11928-11937]. The rates of dissociation of FA from SUV were evaluated by incorporating FA into donor vesicles and measuring transfer to acceptor vesicles. The transfer was followed by changes in internal pH of either donor or acceptor vesicles with stopped flow (C14:0, C16:0, C17:0, C18:0, C18:1, and C18:2) or on-line (C20:0, C22:0, and C24:0) fluorescence. All FA showed a single-exponential transfer process that was slower than the lower limits established for the rate of flip-flop, with t1/2 of dissociation ranging from 20 ms for C14:0 to 1900 s for C24:0. The pseudo-unimolecular rate constant (koff) for dissociation of C14:0 to C26:0 showed a 10-fold decrea...Continue Reading

References

Jan 1, 1989·Annual Review of Nutrition·B J PotterP D Berk
Jan 1, 1995·Progress in Lipid Research·J H Veerkamp, R G Maatman

❮ Previous
Next ❯

Citations

Mar 16, 2007·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Jacquelyn A Thomas, F R Rana
Mar 1, 2000·Lipids·L G Romano-FontesJ Procopio
Sep 29, 2007·Journal of Molecular Neuroscience : MN·Richard A Weisiger
Sep 29, 2007·Journal of Molecular Neuroscience : MN·James A Hamilton, Kellen Brunaldi
Jan 25, 2003·Biochimica Et Biophysica Acta·Alexey AgafonovGalina D Mironova
Dec 7, 2002·Progress in Lipid Research·L Kunst, A L Samuels
Sep 2, 1999·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J A Hamilton
May 4, 1999·The International Journal of Biochemistry & Cell Biology·F A Van NieuwenhovenJ F Glatz
Mar 24, 2012·Animal : an International Journal of Animal Bioscience·D GruffatD Bauchart
Mar 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Itay Budin, Jack W Szostak
Mar 12, 2008·The Journal of Biological Chemistry·Victor A DroverMichael C Phillips
Mar 3, 2010·Annual Review of Biophysics·Itay Budin, Jack W Szostak
Jan 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Donald M Small
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Irene A Chen, Jack W Szostak
Feb 19, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Lalit KumarAmit Kumar Jain
Jun 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Michael D HardyNeal K Devaraj
Nov 23, 2007·Prostaglandins, Leukotrienes, and Essential Fatty Acids·James A Hamilton
Oct 8, 2004·Journal of Biomedical Materials Research. Part a·Xiangying GuanGeorge H Nancollas
Jul 17, 2003·Acta Physiologica Scandinavica·H J Pownall, J A Hamilton
Jan 28, 2006·Chembiochem : a European Journal of Chemical Biology·Marlon J HinnerPeter Fromherz
Mar 18, 2011·Angewandte Chemie·Christophe B MinkenbergJan H van Esch
Jan 3, 2007·Biochimica Et Biophysica Acta·Shelley M Elvington, J Wylie Nichols
Aug 10, 2004·Biophysical Journal·Irene A Chen, Jack W Szostak
Jun 24, 2004·Chemistry and Physics of Lipids·Laura Rabinovich-GuilattCatherine Dubernet
Jul 11, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Frits Kamp, James A Hamilton
Aug 23, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Alfred FahrMathew L S Leigh
Jun 28, 2005·Life Sciences·Sherrye T GlaserDale G Deutsch
Jan 3, 2006·American Journal of Ophthalmology·Liliana WernerNick Mamalis
Apr 20, 2016·Biochimica Et Biophysica Acta·Jan F C GlatzJoost J F P Luiken
Apr 3, 2014·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Jan F C Glatz, Joost J F P Luiken
Apr 9, 1998·Biophysical Journal·J B Massey, H J Pownall
Feb 7, 2008·Annual Review of Plant Biology·Lacey SamuelsReinhard Jetter
Jul 2, 2002·Journal of Lipid Research·Ji-Kyung ChoiJames A Hamilton
Sep 18, 2002·Journal of Lipid Research·Jet K HoJames A Hamilton
Jun 17, 2004·The Journal of Biological Chemistry·J Patrick Kampf, Alan M Kleinfeld
Dec 22, 2017·The Journal of Membrane Biology·Filipe M Coreta-GomesMaria J Moreno

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.