PMID: 8948629Nov 15, 1996Paper

Dissociation of long-chain duplex RNA can occur via strand displacement in vitro: biological implications

Nucleic Acids Research
M HomannGeorg Sczakiel

Abstract

Hammerhead ribozymes with long antisense flanks (>50 bases) have been used successfully to inhibit replication of human immunodeficiency virus type 1 (HIV-1) in living cells. To explain their increased efficacy versus antisense controls or catalytically inactive derivatives, one can consider dissociation of the ribozyme-product complex to allow a complete catalytic cycle. In this work we investigated the dissociation of a double-stranded RNA with 56 bp in vitro. Dissociation was observed in the presence of single-stranded RNA with sequence complementarity to one of the duplex strands. A displacement reaction between RNA single strands and the duplex, but not simple dissociation, was strongly suggested by the concentration dependence of this process, the influence of additional non-complementary sequences on the single strand and by the unusually low Arrhenius activation energy. The strand displacement reaction was slow in vitro at 37 degrees C and physiological ionic strength, but was increased to k approximately 10(3)-10(4)/M/s (approximately 10(4)-fold) at higher temperatures by cetyltrimethylammonium bromide. This compound is thought to enhance non-sequence-specific association of nucleic acids in a mechanistically similar w...Continue Reading

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Citations

Nov 15, 2011·Journal of Molecular Evolution·Rei OhmoriTan Inoue
Aug 1, 1997·Antisense & Nucleic Acid Drug Development·G Sczakiel
Oct 8, 2009·Nucleic Acids Research·Grace S TanMarianthi Kiriakidou
Sep 11, 2013·Nucleic Acids Research·Niranjan SrinivasErik Winfree
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·P TranqueV P Mauro
Nov 6, 2012·Colloids and Surfaces. B, Biointerfaces·Bryan A BakerValeria T Milam
Nov 19, 2015·Nucleic Acids Research·Simon DornseiferTobias Restle
Mar 23, 2004·Journal of Molecular Biology·Michele R S HargittaiKarin Musier-Forsyth
Mar 13, 2015·Biophysical Journal·Petr ŠulcArd A Louis
Jul 13, 2018·Journal of the Royal Society, Interface·Tomislav PlesaRadek Erban
Jun 15, 2018·Nature Communications·Ranajay SahaIrene A Chen

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