Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Reproduction : the Official Journal of the Society for the Study of Fertility
A A Fouladi-Nashta, K H S Campbell

Abstract

Follicles of 4-8 mm in diameter were dissected from ovaries and cultured in Waymouth culture medium in the presence or absence of insulin (5 mug/ml) at 39 degrees C in a humidified atmosphere of 45% O2, 5% CO2 and 50% N2 for 24 h. Following follicle culture, the oocytes were collected and examined for developmental potential, total protein profile and ultrastructural aspects. Oocytes aspirated directly from follicles of the same size were used as controls. Addition of insulin to the follicle culture medium significantly reduced expression of the low molecular weight insulin-like growth factor-binding proteins (IGFBPs) in the follicular fluid, and significantly reduced the cleavage rate of subsequently matured and fertilised oocytes (0.52 vs 0.61). However, there were no differences in the proportion of cleaved embryos which developed to the blastocyst stage (0.30 vs 0.28), nor embryo quality as assessed by total cell number (137 +/- 8.53 vs 124.6 +/- 6.95). The total protein profiles of immature oocytes recovered after 24 h of follicle culture were compared by PAGE. There were marked differences between the two groups, unmatured oocytes recovered from the insulin-positive follicle group showed a protein pattern similar to that ...Continue Reading

References

Apr 1, 1975·Journal of Reproduction and Fertility·A Tsafriri, C P Channing
Jan 1, 1992·Reproduction, Fertility, and Development·P L KayeM B Harvey
Nov 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L V RaoS Heyner
Jan 11, 1991·Reproduction, Fertility, and Development·J G ThompsonH R Tervit
Jul 1, 1985·Journal of Reproduction and Fertility·K G OsteenC P Channing
Mar 1, 1988·Endocrinology·M B Harvey, P L Kaye
May 1, 1987·Endocrine Reviews·L Poretsky, M F Kalin
Nov 1, 1985·The Journal of Clinical Endocrinology and Metabolism·M P DiamondK G Osteen
May 1, 1984·Molecular and Cellular Endocrinology·J B Davoren, A J Hsueh
Nov 23, 1981·Life Sciences·D S Straus
Apr 1, 1994·Biology of Reproduction·A J WatsonR F Seamark
Jul 1, 1995·Domestic Animal Endocrinology·L J Spicer, S E Echternkamp
Apr 1, 1993·British Journal of Obstetrics and Gynaecology·M TulppalaO Ylikorkala
Oct 1, 1996·Biology of Reproduction·R L de la SotaW W Thatcher
Jun 20, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·E M FergusonM G Hunter
Jul 25, 1958·The Journal of Biophysical and Biochemical Cytology·M L WATSON
Sep 24, 2004·Best Practice & Research. Clinical Obstetrics & Gynaecology·Zephne M van der Spuy, Silke J Dyer
Mar 5, 2005·Reproduction, Fertility, and Development·Daniel A DumesicDavid H Abbott

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Citations

Nov 18, 2011·Cell and Tissue Research·R N ChavesJ R Figueiredo
Apr 30, 2013·Molecular Human Reproduction·Waleed F A MareiAli A Fouladi-Nashta
Jul 28, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Ali A Fouladi-NashtaRobert Webb
Mar 11, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Waleed F MareiAli A Fouladi-Nashta
Feb 1, 2014·Reproductive Biology and Endocrinology : RB&E·Song-yi Jung, Scott T Willard
Jan 9, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·P C GarnsworthyR Webb
Dec 3, 2014·Animal Reproduction Science·J M Carrera-ChávezC F Aréchiga-Flores
Aug 21, 2010·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·G Taru SharmaG Sai Kumar
Mar 10, 2016·In Vitro Cellular & Developmental Biology. Animal·Amar NathRituraj Konwar
Jun 11, 2014·Zygote : the Biology of Gametes and Early Embryos·Gustavo Bruno MotaAlzira Amélia Martins Rosa E Silva
Mar 9, 2007·Biology of Reproduction·Ali A Fouladi-NashtaRobert Webb
Jul 10, 2009·Biology of Reproduction·Waleed F MareiAli A Fouladi-Nashta
Feb 29, 2016·Reproduction, Fertility, and Development·Denise LaskowskiRenée Båge
Feb 15, 2019·International Journal of Veterinary Science and Medicine·Waleed F A MareiAli Fouladi-Nashta
Feb 15, 2019·International Journal of Veterinary Science and Medicine·Yaşar AkarMuhammad Khalıd
Jul 8, 2020·Animals : an Open Access Journal From MDPI·Junnan MaAriette T M van Knegsel

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