PMID: 1203256Dec 19, 1975Paper

Dissociation of the DNA replicase system of bovine lymphocyte nuclei

Biochimica Et Biophysica Acta
L R Thompson, G C Mueller

Abstract

Exposure of S-phase nuclei or subnuclear preparations from phytohemagglutinin-stimulated bovine lymphocytes to 0.02 M ATP caused an immediate and almost total loss of their ability to replicate DNA in vitro. Other ribonucleoside and deoxyribonucleoside triphosphates caused a similar inhibition of DNA replication. Levels of ATP which inhibit replication cause the release of DNA polymerases alpha and beta and small pieces of DNA from these nuclei. This release occurs both at 4 and 37 degrees C. The data support the conclusion that high levels of ATP or other nucleoside triphosphates inhibit DNA replication in nuclei by dissolution of the DNA replication complex. The limited success in reconstitution of the DNA replicase complexes is discussed.

References

Feb 10, 1975·Biochimica Et Biophysica Acta·L R Thompson, G C Mueller
Feb 11, 1971·Biochimica Et Biophysica Acta·E K Schandl, J H Taylor
Sep 1, 1973·The Journal of Cell Biology·R O Williams, L A Loeb
Jul 2, 1971·Biochemical and Biophysical Research Communications·J P SlaterL A Loeb
Nov 12, 1970·Biochimica Et Biophysica Acta·C TengR Roychoudhury
Jan 25, 1968·Biochemical and Biophysical Research Communications·L R Thompson, B J McCarthy
Jul 23, 1968·Biochimica Et Biophysica Acta·D L Friedman, G C Mueller
Apr 1, 1969·Experimental Cell Research·D Lagunoff
Nov 8, 1965·Biochemical and Biophysical Research Communications·E N Brewer, H P Rusch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochimica Et Biophysica Acta
N A Berger, E S Johnson
Proceedings of the National Academy of Sciences of the United States of America
B R FridlanderJ Mordoh
Proceedings of the National Academy of Sciences of the United States of America
A AdamsG Klein
© 2022 Meta ULC. All rights reserved