Dissolved carbon dioxide accumulation in a large scale and high density production of TGFβ receptor with baculovirus infected Sf-9 cells.

Cytotechnology
A GarnierA Kamen

Abstract

Production of a TGFβ receptor with high density baculovirus infected Sf-9 cells (7×10(6)cells ml(-1)) served as a test run for a retrofitted 150 L microbial fermentor. The entire 110 L batch run was performed in serum free medium, with an addition of a concentrated amino acid and yeastolate mixture at the time of infection. This addition strategy has been proven effective at a small scale by enabling cultures to maintain maximum product yield. In the bioreactor however, while cellular growth was comparable to that of the smaller scale control, TGFβ receptor production was three fold below the control. To minimize the mechanical stress, low flow rate of pure oxygen was used to control the dissolved oxygen at 40%. As a consequence, it seems that this aeration strategy involved an accumulation of dissolved carbon dioxide that in turn inhibited the protein production. A model has been developed that estimated the CO(2) partial pressure in the culture to be in the vicinity of 0.15 atm. The effect of dissolved CO(2) at this concentration has been assessed at smaller scale for TGFβ receptor and β-gal expression, in controlled atmosphere incubators.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 15, 1992·Biotechnology and Bioengineering·D A Lindsay, M J Betenbaugh
Jun 20, 1992·Biotechnology and Bioengineering·J E LazarteC Nicolau
Apr 15, 1994·Biotechnology and Bioengineering·A W CaronB Massie

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Citations

Nov 13, 2008·Cytotechnology·Alvin W Nienow
Apr 1, 1997·Current Opinion in Biotechnology·W S Hu, J G Aunins
Nov 13, 2008·Cytotechnology·David M Marks
Oct 11, 2011·Biotechnology Advances·Jean-Christophe DrugmandSpiros N Agathos
Dec 15, 2007·Biotechnology and Bioengineering·Jinpian DiaoMichael Louis Shuler
Dec 16, 2006·Biotechnology and Bioengineering·Chetan T GoudarKonstantin B Konstantinov
Nov 23, 2013·Biotechnology Progress·A Contreras-GómezY Chisti
Jul 14, 2010·MAbs·Feng LiAshraf Amanullah
Jan 20, 2016·Biotechnology Progress·Sucheta G Vajrala, David W Murhammer
May 10, 2002·Biotechnology and Bioengineering·Vivian M deZengotitaWilliam M Miller
Apr 1, 1999·Biotechnology and Bioengineering·P E CruzM J Carrondo

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