Dissolved molecular hydrogen (H2 ) in Peritoneal Dialysis (PD) solutions preserves mesothelial cells and peritoneal membrane integrity

BMC Nephrology
Masaaki NakayamaSadayoshi Ito

Abstract

Peritoneal dialysis (PD) is used as renal replacement therapy in patients with end-stage kidney disease. However, peritoneal membrane failure remains problematic and constitutes a critical cause of PD discontinuation. Recent studies have revealed the unique biological action of molecular hydrogen (H2) as an anti-oxidant, which ameliorates tissue injury. In the present study, we aimed to examine the effects of H2 on the peritoneal membrane of experimental PD rats. Eight-week-old male Sprague-Dawley rats were divided into the following groups (n = 8-11 each) receiving different test solutions: control group (no treatment), PD group (commercially available lactate-based neutral 2.5% glucose PD solution), and H2PD group (PD solution with dissolved H2 at 400 ppb). Furthermore, the influence of iron (FeCl3: 5 μM: inducer of oxidative cellular injury) in the respective PD solutions was also examined (Fe-PD and Fe-H2PD groups). The H2PD solution was manufactured by bathing a PD bag in H2-oversaturated water created by electrolysis of the water. Twenty mL of the test solutions were intraperitoneally injected once a day for 10 days. Parietal peritoneum samples and cells collected from the peritoneal surface following treatment with tryps...Continue Reading

References

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Citations

Jan 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hongtao LuZhiyong Guo
Nov 21, 2019·International Journal of Molecular Sciences·Rajesh M JagirdarVassilios Liakopoulos
Aug 8, 2021·International Journal of Molecular Sciences·Mario BonominiGiovanni Gambaro

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Methods Mentioned

BETA
light microscopy
PCR

Software Mentioned

Sigma plot
Microarray
ImageJ
Filgen

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