Distal-less homeobox 5 inhibits adipogenic differentiation through the down-regulation of peroxisome proliferator-activated receptor γ expression

Journal of Cellular Physiology
Hye-Lim LeeJeong-Hwa Baek

Abstract

Distal-less homeobox 5 (Dlx5) is a positive regulator of osteoblast differentiation that contains a homeobox domain. Because there are possible reciprocal relationships between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (MSCs), we examined the regulatory role of Dlx5 in adipogenic differentiation in this study. Adipogenic stimuli suppressed the expression levels of Dlx5 mRNA in mouse bone marrow stromal cells. Over-expression of Dlx5 inhibited adipogenic differentiation in human bone marrow MSCs and 3T3-L1 preadipocytic cells whereas knockdown of Dlx5 enhanced adipogenic differentiation in 3T3-L1 cells. Over-expression of Dlx5 suppressed the expression of adipogenic marker genes, including CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). Dlx5-mediated suppression of adipogenic differentiation was overcome by over-expression of PPARγ but not by that of cAMP response element binding protein (CREB) or C/EBPα. Dlx5 decreased the transcriptional activity of CREB and C/EBPα in a dose-dependent manner. Dlx5 directly bound to CREB and C/EBPα and prevented them from binding to and subsequently transactivating the PPARγ promoter. These results suggest...Continue Reading

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Citations

Jan 20, 2018·Stem Cells International·June Seok HeoHyun Ok Kim
Nov 27, 2014·Journal of Cellular Biochemistry·Abdul S QadirJeong-Hwa Baek
Apr 16, 2014·Journal of Cellular Biochemistry·Abdul S QadirJeong-Hwa Baek
Jun 10, 2017·International Journal of Molecular Sciences·Hirotaka Yoshioka, Yuji Yoshiko
Sep 3, 2021·Biological & Pharmaceutical Bulletin·Chae Yul KimKwang Youl Lee

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