PMID: 8962064Dec 10, 1996Paper

Distamycin A modulates the sequence specificity of DNA alkylation by duocarmycin A

Proceedings of the National Academy of Sciences of the United States of America
Hiroshi SugiyamaA H Wang

Abstract

Duocarmycin A (Duo) normally alkylates adenine N3 at the 3' end of A + T-rich sequences in DNA. The efficient adenine alkylation by Duo is achieved by its monomeric binding to the DNA minor groove. The addition of another minor groove binder, distamycin A (Dist), dramatically modulates the site of DNA alkylation by Duo, and the alkylation switches preferentially to G residues in G + C-rich sequences. HPLC product analysis using oligonucleotides revealed a highly efficient G-N3 alkylation via the cooperative binding of a heterodimer between Duo and Dist to the minor groove. The three-dimensional structure of the ternary alkylated complex of Duo/Dist/d(CAGGTGGT).d(ACCACCTG) has been determined by nuclear Overhauser effect (NOE)-restrained refinement using 750 MHz two-dimensional NOE spectroscopy data. The refined NMR structure fully explains the sequence requirement of such modulated alkylations. This is the first demonstration of Duo DNA alkylation through cooperative binding with another structurally different natural product, and it suggests a promising new way to alter or modify the DNA alkylation selectivity in a predictable manner.

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M MrksichP B Dervan
Jan 1, 1989·Annual Review of Genetics·S Adhya
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J G Pelton, D E Wemmer
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M CollA Rich
Mar 1, 1994·Nature Structural Biology·X ChenM Sundaralingam
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·D L Boger, D S Johnson
Jun 1, 1994·Current Opinion in Cell Biology·J A Goodrich, R Tjian
Oct 28, 1994·Science·B H GeierstangerD E Wemmer
Jan 1, 1993·Cold Spring Harbor Symposia on Quantitative Biology·N Tanese, R Tjian
Apr 1, 1994·Current Opinion in Genetics & Development·R Hori, M Carey
May 6, 1994·Science·C Anderson
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D S GoodsellR E Dickerson
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H Robinson, A H Wang
Apr 1, 1996·Nature Structural Biology·B H GeierstangerD E Wemmer

❮ Previous
Next ❯

Citations

Feb 24, 2011·Journal of Fluorescence·Malaichamy Ilanchelian, Ramasamy Ramaraj
Nov 27, 1999·Pharmacology & Therapeutics·X L Yang, A H Wang
Nov 19, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Min ChenHiroki Nagase
Dec 30, 2009·Anti-cancer Drugs·Ken-Ichi ShinoharaHiroshi Sugiyama
May 1, 2013·Biological & Pharmaceutical Bulletin·Akifumi IguchiKazufumi Shimizu
May 30, 2013·Biophysical Chemistry·Marie-Virginie SalviaJohn A Parkinson
Apr 11, 2007·Bioscience, Biotechnology, and Biochemistry·Masahiko YamamotoNaomichi Baba
Aug 12, 2010·Journal of Nucleic Acids·Etsuko KawashimaKazuo Kamaike
Feb 27, 2007·The International Journal of Biochemistry & Cell Biology·Mikako HirotaHirobumi Teraoka
May 17, 2012·Biotechnology Journal·Ganesh N Pandian, Hiroshi Sugiyama
Aug 30, 2005·Bioorganic & Medicinal Chemistry Letters·Kiyohiko KawaiTetsuro Majima
Apr 5, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bruna L AurasBernardo A Iglesias
Nov 15, 2007·Chemistry : a European Journal·Shunta SasakiHiroshi Sugiyama
Jun 20, 2019·Journal of Cellular Physiology·Sajad YaghoubiMeghdad Abdollahpour-Alitappeh
Jun 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Keiko TakagiNoboru Fukuda
Dec 21, 2006·Accounts of Chemical Research·Toshikazu Bando, Hiroshi Sugiyama
Jan 11, 2005·Archives of Biochemistry and Biophysics·Takuya IwamotoShosuke Kawanishi
Jan 13, 2018·Chemical Reviews·Alicia J AngelbelloMatthew D Disney
Jul 24, 2018·Chemical Research in Toxicology·Owen ZacariasElise Champeil
Oct 6, 2005·Journal of the American Chemical Society·Toshikazu BandoHiroshi Sugiyama
Mar 20, 2003·Journal of the American Chemical Society·Toshikazu BandoHiroshi Sugiyama
Jul 22, 2004·Journal of the American Chemical Society·Toshikazu BandoHiroshi Sugiyama
Sep 18, 2021·Cancer Science·Hiroki NagaseJason Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved