PMID: 6162473Dec 23, 1980Paper

Distances between 3' ends of ribosomal ribonucleic acids reassembled into Escherichia coli ribosomes

Biochemistry
O W OdomB Hardesty

Abstract

The three ribonucleic acids (RNAs) from Escherichia coli ribosomes were isolated and then labeled at their 3' ends by oxidation with periodate followed by reaction with thiosemicarbazides of fluorescein or eosin. Ribosomal subunits reconstituted with the labeled RNAs were active for polyphenylalanine synthesis. The distances between the 3' ends of the RNAs in 70S ribosomes were estimated by nonradiative energy transfer from fluorescein to eosin. The percentage of energy transfer was calculated from the decrease in fluorescence lifetime of fluorescein in the quenched sample compared to the unquenched sample. Fluorescence lifetime was measured in real time by using a mode-locked laser for excitation and a high-speed electrostatic photomultiplier tube for detection of fluorescence. The distances between fluorophores attached to the 3' ends of 16S RNA and 5S RNA or 23S RNA were estimated to be about 55 and 71 A, respectively. The corresponding distance between the 5S RNA and 23S RNA was too large to be measured reliably with the available probes but was estimated to be greater than 65 A. Comparison of the quantum yields of the labeled RNAs free in solution and reconstituted into ribosomal subunits suggests that the 3' end of 16S RN...Continue Reading

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Citations

Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
Aug 27, 1990·Biochimica Et Biophysica Acta·B HardestyO W Odom
Apr 2, 2013·Nature Structural & Molecular Biology·Chunlai ChenYale E Goldman
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M Stöffler-MeilickeB Hardesty
Jun 5, 2012·The Journal of Biological Chemistry·Cordula BöhmH Ulrich Göringer
Nov 15, 1996·Nucleic Acids Research·D Proudnikov, A Mirzabekov
Jan 14, 2005·Nucleic Acids Research·Magdalena DorywalskaJoseph D Puglisi
Dec 1, 1983·European Journal of Biochemistry·K G SteinhäuserB Epe
May 8, 1991·European Journal of Biochemistry·W KudlickiB K Iskakov
Jun 15, 1991·European Journal of Biochemistry·O W OdomB Hardesty
Jun 4, 2008·Annual Review of Biochemistry·R Andrew MarshallJoseph D Puglisi
Sep 18, 2003·RNA·Francesco VanziBarry S Cooperman
Jun 14, 2012·PloS One·Benjamin StevensYale E Goldman
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B EpeP Woolley
Mar 31, 2007·Angewandte Chemie·Dana A Baum, Scott K Silverman
Oct 14, 2011·Angewandte Chemie·On Yi WongScott K Silverman
Mar 13, 2001·Methods : a Companion to Methods in Enzymology·D Klostermeier, D P Millar
Apr 20, 1999·Methods : a Companion to Methods in Enzymology·P Z Qin, A M Pyle
Jan 1, 1997·Folding & Design·W KudlickiB Hardesty
Apr 1, 1984·Archives of Biochemistry and Biophysics·O W OdomB Hardesty
Dec 6, 1996·The Journal of Biological Chemistry·W KudlickiB Hardesty
Oct 1, 1982·European Journal of Biochemistry·K G SteinhäuserJ Dijk

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