Distilbene derivative as a new environment-sensitive bifunctional ligand for the possible induction of serum protein aggregation: a spectroscopic investigation and potential consequences

Langmuir : the ACS Journal of Surfaces and Colloids
Shubhashis DattaMintu Halder

Abstract

The photophysical properties of a new distilbene fluorophore, DPDB, belonging to the conjugated polyene family is found to be well modulated with the variation of the microenvironment. Compared to the ground state, the excited-state photophysical properties of the fluorophore have been altered to larger extents with the variation of polarity and the hydrogen-bonding nature of solvents. The change in the fluorescence intensity of DPDB shows a nice correlation with the aggregation behavior of different surfactants which have been utilized for the determination of the CMC of surfactants. The distribution of DPDB is found to be higher in nonionic micelles. On the other hand, DPDB specifically binds the subdomain IB cavity of serum albumin with a stronger binding ability with HSA compared to BSA. DPDB behaves like a bivalent (bifunctional) ligand and forms a complex of 2:1 stoichiometry with serum albumins. Dynamic light scattering and circular dichroism measurements indicate that DPDB favors the association of serum albumin molecules, promoting their preaggregation state. Aggregation is an important phenomenon and is known to be initiated by heat, extreme pH conditions, very high ionic strength, surfactants, metal ions, and so fort...Continue Reading

References

Jan 1, 1975·Topics in Current Chemistry·D Döpp
Jun 9, 1989·Science·D C CarterT Y Miller
Jan 1, 1985·Advances in Protein Chemistry·T Peters
Jan 1, 1982·Methods in Enzymology·B J Litman, Y Barenholz
Jan 1, 1994·Advances in Protein Chemistry·D C Carter, J X Ho
May 1, 1998·Pharmaceutical Research·T KosaM Otagiri
Nov 1, 2000·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·E L Gelamo, M Tabak
Oct 24, 2002·Biochemical Pharmacology·Nicole A KratochwilPaul R Gerber
Jul 9, 2003·BMC Structural Biology·Patricia A ZunszainStephen Curry
Aug 28, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Massimo Stefani, Christopher M Dobson
Feb 14, 2004·Biophysical Chemistry·Valeria MilitelloMaurizio Leone
May 25, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fernanda G De FeliceSérgio T Ferreira
Feb 26, 2005·Biophysical Journal·Changxia SunShufang Liu
Jul 23, 2005·The Journal of Biological Chemistry·Jason G S HoKenneth K-S Ng
Sep 20, 2005·Journal of Molecular Biology·Jamie GhumanStephen Curry
Jun 26, 2007·Biomacromolecules·Valeria CastellettoPaula Jauregi
Jul 8, 2008·Journal of Molecular Biology·Patricia A ZunszainStephen Curry
Jan 20, 2009·Journal of Colloid and Interface Science·Yinyan ZhaoMichael T Harris
Mar 2, 2010·Nature Reviews. Drug Discovery·Adriano Aguzzi, Tracy O'Connor
Sep 28, 2010·Bioorganic & Medicinal Chemistry·David ButtarHolger Steuber
Oct 5, 2011·The Journal of Physical Chemistry. B·Afshin Asadzadeh ShahirHussein Gharibi
Mar 1, 2012·Organic & Biomolecular Chemistry·Prithiba MitraDipakranjan Mal
May 11, 2013·Journal of Photochemistry and Photobiology. B, Biology·Shubhashis DattaMintu Halder

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.