Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells

Cancers
Fabio ContuSabine Mai

Abstract

Classical Hodgkin's lymphoma (cHL) is a B-Cell lymphoma comprised of mononuclear Hodgkin cells (H) and bi- to multi-nucleated Reed-Sternberg (RS) cells. Previous studies revealed that H and RS cells express lamin A/C, a component of the lamina of the nuclear matrix. Since no information was available about the three-dimensional (3D) expression patterns of lamin A/C in H and RS cells, we analyzed the 3D spatial organization of lamin in such cells, using 3D fluorescent microscopy. H and RS cells from cHL derived cell lines stained positive for lamin A/C, in contrast to peripheral blood lymphocytes (PBLs), in which the lamin A/C protein was not detected or weak, although its presence could be transiently increased with lymphocyte activation by lipopolysaccharide (LPS). Most importantly, in H and RS cells, the regular homogeneous and spherically shaped lamin A/C pattern, identified in activated lymphocytes, was absent. Instead, in H and RS cells, lamin staining showed internal lamin A/C structures, subdividing the nuclei into two or more smaller compartments. Analysis of pre-treatment cHL patients' samples replicated the lamin patterns identified in cHL cell lines. We conclude that the investigation of lamin A/C protein could be a ...Continue Reading

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Citations

Dec 12, 2018·Genes, Chromosomes & Cancer·Sabine Mai
Feb 27, 2020·Journal of Clinical Medicine·Aline Rangel-PozzoSabine Mai
Aug 29, 2020·International Journal of Molecular Sciences·Angela SaezJose M Gonzalez-Granado
Sep 16, 2020·International Journal of Molecular Sciences·Marc BienzHans Knecht
Dec 12, 2018·Genes, Chromosomes & Cancer·Anat VivanteYuval Garini
Dec 16, 2020·Cancers·Niina Dubik, Sabine Mai

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Methods Mentioned

BETA
biopsy
transfection

Software Mentioned

ScanView
SpotScan
ZEN Blue
TeloView
DIPimage toolbox for Matlab
NIH Image J
ImageJ
GenASIs
TANGO
Draw Spline

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