Distinct adhesion of probiotic strain Lactobacillus casei ATCC 393 to rat intestinal mucosa

Anaerobe
Georgia SaxamiAlex Galanis

Abstract

Adhesion to the intestine represents a critical parameter for probiotic action. In this study, the adhesion ability of Lactobacillus casei ATCC 393 to the gastrointestinal tract of Wistar rats was examined after single and daily administration of fermented milk containing either free or immobilized cells on apple pieces. The adhesion of the probiotic cells at the large intestine (cecum and colon) was recorded at levels ≥6 logCFU/g (suggested minimum levels for conferring a probiotic effect) following daily administration for 7 days by combining microbiological and strain-specific multiplex PCR analysis. Single dose administration resulted in slightly reduced counts (5 logCFU/g), while they were lower at the small intestine (duodenum, jejunum, ileum) (≤3 logCFU/g), indicating that adhesion was a targeted process. Of note, the levels of L. casei ATCC 393 were enhanced in the cecal and colon fluids both at single and daily administration of immobilized cells (6 and 7 logCFU/g, respectively). The adhesion of the GI tract was transient and thus daily consumption of probiotic products containing the specific strain is suggested as an important prerequisite for retaining its levels at an effective concentration.

References

Sep 27, 2002·Applied and Environmental Microbiology·Sanna EdelmanTimo K Korhonen
Feb 10, 2004·Applied and Environmental Microbiology·Y K LeeS Salminen
Feb 10, 2004·Applied and Environmental Microbiology·Nana ValeurKarin Ladefoged
Dec 21, 2004·Journal of Internal Medicine·A Sullivan, C E Nord
Nov 5, 2005·Applied and Environmental Microbiology·Je-Ruei LiuXin Zhao
Apr 20, 2006·Letters in Applied Microbiology·S S ChoiS H Kim
Jan 10, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Maija Saxelin
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Matti KankainenWillem M de Vos

❮ Previous
Next ❯

Citations

Nov 30, 2013·Journal of Nutrition and Metabolism·Gregoria MitropoulouYiannis Kourkoutas
Jan 29, 2014·TheScientificWorldJournal·Rastislav SalajAlojz Bomba
Mar 19, 2013·Journal of Dairy Science·Marianthi SidiraYiannis Kourkoutas
Jun 22, 2017·Current Microbiology·Valentini SantarmakiPetros Ypsilantis
Mar 21, 2017·Probiotics and Antimicrobial Proteins·Cesar Antonio Irecta-NájeraAnne Santerre
Dec 17, 2020·Biomedicines·Stavros PlessasEugenia Bezirtzoglou
Apr 26, 2020·Microbial Pathogenesis·Juanjuan LiuJiakui Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.