Distinct arginase isoforms expressed in primary and transformed macrophages: regulation by oxygen tension

The American Journal of Physiology
C A LouisJ E Albina

Abstract

Experiments were performed to identify arginase isoforms expressed in primary and transformed rodent macrophages and to determine the molecular mechanisms for the previously observed increase in arginase activity in macrophages cultured in hypoxia or anoxia. Results demonstrate the following: 1) mRNA and protein for hepatic-type AI arginase are expressed in primary cultures of rat and mouse peritoneal macrophages and are enhanced seven- and nine-fold, respectively, by lipopolysaccharide (LPS). 2) mRNA for extrahepatic-type AII arginase is constitutively expressed in mouse, but not rat, peritoneal macrophages and is detected in RAW264.7 cells after LPS treatment; neither J774A.1 nor P388D1 cells contain arginase mRNA. 3) AI arginase mRNA, arginase activity in cell lysates, and L-arginine flux through arginase in intact cells are all increased in rat wound-derived and mouse peritoneal macrophages by hypoxic or anoxic culture; AII arginase mRNA is, in contrast, suppressed > 50% by O2 deprivation. 4) Expression of the L-arginine transporter mCAT-2 is increased greater than twofold by reduced O2 culture. These results demonstrate substantial variability in arginase isoform expression among primary and transformed rodent macrophages....Continue Reading

References

Sep 1, 1977·The Journal of Experimental Medicine·J T KungD W Talmage
Jun 1, 1979·British Journal of Cancer·G A CurrieL Cifuentes
Feb 25, 1992·Nucleic Acids Research·I Oberbäumer
Apr 1, 1991·The Journal of Surgical Research·J E Albina, W L Henry
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M A GoldbergH F Bunn
Nov 26, 1986·Biochemical and Biophysical Research Communications·G J DizikesS D Cederbaum
Jan 1, 1980·Journal of Immunological Methods·A S Russell, U T Ruegg
Mar 1, 1980·Proceedings of the Society for Experimental Biology and Medicine·P C Chen, J D Broome
May 25, 1995·Biochemical and Biophysical Research Communications·W W WangL J Ignarro
Sep 22, 1995·Cell·C Nathan
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·K D FinleyC L MacLeod
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Jan 20, 1995·The Journal of Biological Chemistry·G L Wang, G L Semenza
Jul 26, 1996·The Journal of Biological Chemistry·B H JiangG L Semenza
May 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C P JenkinsonS D Cederbaum
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·P W HochachkaS C Land

❮ Previous
Next ❯

Citations

Nov 18, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·D Kepka-LenhartS M Morris
Apr 20, 2007·American Journal of Physiology. Cell Physiology·Leif D NelinYusen Liu
Sep 24, 2010·American Journal of Physiology. Cell Physiology·Karina KrotovaSergey Zharikov
Aug 16, 2019·Physiological Reviews·Carla Riera-DomingoMassimiliano Mazzone
May 21, 2004·American Journal of Respiratory and Critical Care Medicine·Ignacio FajardoGunnar Pejler
Feb 24, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Louis G ChicoineLeif D Nelin
Jul 5, 2005·American Journal of Respiratory Cell and Molecular Biology·Leif D NelinYusen Liu
Jun 11, 2002·Annual Review of Nutrition·Sidney M Morris
Feb 15, 2008·American Journal of Physiology. Cell Physiology·Mary A RobinsonCynthia M Otto
Jan 16, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Scott HorowitzParvaneh Rafiee
Apr 8, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Motoyoshi EndoTomomi Gotoh
Oct 6, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Bernadette ChenLeif D Nelin
Sep 15, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Weiling XuSerpil C Erzurum
Feb 24, 2001·American Journal of Physiology. Cell Physiology·C M Otto, J E Baumgardner
Oct 7, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Irina A IonovaGalen M Pieper
Aug 29, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Keita MikiRussell L Delude
May 28, 2020·Journal of Cellular Physiology·Seham A Abd El-AleemLaiche Djouhri
Nov 8, 2001·American Journal of Physiology. Cell Physiology·J E AlbinaJ S Reichner
May 16, 2018·Biomedicines·Ewelina Krzywinska, Christian Stockmann
Dec 4, 2019·Intensive Care Medicine Experimental·Simon Lambden
Dec 9, 1998·Kidney International·N BankP C Singhal
May 27, 2021·Cellular and Molecular Life Sciences : CMLS·Adrià-Arnau Martí I Líndez, Walter Reith
Oct 1, 2011·Free Radical Biology & Medicine·Mary A RobinsonCynthia M Otto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.