Distinct behaviors of infectious F-specific RNA coliphage genogroups at a wastewater treatment plant

The Science of the Total Environment
Eiji HaramotoMikie Otagiri

Abstract

The present study aimed to determine the differences in the behaviors of four F-specific RNA (F-RNA) coliphage genogroups (GI-GIV) during wastewater treatment. Raw sewage, aeration tank effluent, secondary-treated sewage, and return activated sludge were collected from a wastewater treatment plant in Japan at monthly intervals between March and December 2011 (n=10 each). F-specific coliphages were detected by plaque assay in all tested samples, with a concentration ranging from -0.10 to 3.66 log10 plaque-forming units/ml. Subsequently, eight plaques were isolated from each sample, followed by genogroup-specific reverse-transcription quantitative PCR (qPCR) for F-RNA coliphages and qPCR for F-specific DNA (F-DNA) coliphages. GI F-RNA coliphages were the most abundant in the secondary-treated sewage samples (73% of the plaque isolates), while GII F-RNA coliphages were the most abundant in the other three sample types (41-81%, depending on sample type). Based on the results of the quantification and genotyping, the annual mean concentrations of each F-specific coliphage type were calculated, and their reduction ratios during wastewater treatment were compared with those of indicator bacteria (total coliforms and Escherichia coli) ...Continue Reading

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Citations

Mar 18, 2017·Letters in Applied Microbiology·B R McMinnA Korajkic
May 22, 2016·Applied and Environmental Microbiology·Akihiko HataHiroaki Tanaka
Sep 24, 2018·Journal of Applied Microbiology·C García-AljaroF Lucena
Oct 9, 2016·Folia Microbiologica·Sonika SharmaMohan G Vairale
Jul 3, 2020·Food and Environmental Virology·Sarmila TandukarEiji Haramoto
Jul 16, 2020·Food and Environmental Virology·JaeYoon LeeGwangPyo Ko
Aug 5, 2018·Environmental Science and Pollution Research International·Kyuseon ChoGwangPyo Ko
Dec 10, 2020·Frontiers of Environmental Science & Engineering·Mengzhi JiQiang Li
May 21, 2021·Frontiers in Microbiology·Elisenda BallestéCristina García-Aljaro

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