Distinct biogenesis mechanisms for the water channels MIWC and CHIP28 at the endoplasmic reticulum

Biochemistry
L B ShiA S Verkman

Abstract

MIWC is a 32 kDa mercurial-insensitive water channel [Hasegawa et al. (1994) J. Biol. Chem. 269, 5497-5500] expressed in kidney collecting duct, brain ependymal cells, airways, and other tissues. We showed recently that the homologous water channel CHIP28 spanned the endoplasmic reticulum (ER) membrane 4 times with N- and C-termini in the cytoplasm [Skach et al., (1994) J. Cell Biol. 125, 803-815]. Hydropathy analysis of MIWC indicated up to eight hydrophobic regions (HRs) comprising potential membrane-spanning domains. To determine MIWC transmembrane topology at the ER, 10 cDNA chimeras were constructed which encoded increasing lengths of MIWC upstream from a reporter epitope (prolactin P-domain) at residues 13, 46, 73, 92, 120, 140, 164, 209, 276, and 2097, corresponding to putative polar extramembrane loops in the MIWC sequence. The chimeras were translated cell-free (rabbit reticulocyte lysate+ER-derived microsomes) and in Xenopus oocytes. Peptide chains were labeled with [35S]methionine and immunoprecipitated with a P-domain antibody. Transmembrane topology as determined by protease accessibility of the P-reporter indicated six membrane-spanning domains with N- and C-termini in the cytoplasm. The predicted topology was con...Continue Reading

References

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Citations

Jun 6, 2009·Nature Structural & Molecular Biology·William R Skach
Jul 17, 2007·Nature Structural & Molecular Biology·Teresa M BuckWilliam R Skach
Sep 28, 2005·Nature Structural & Molecular Biology·Heather SadlishWilliam R Skach
Jun 26, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ken-ichi NakahamaYasufumi Shigeyoshi
Sep 12, 2000·Molecular Biology of the Cell·Y LuW R Skach
May 23, 2008·Molecular Biology of the Cell·Jonathan M CraneA S Verkman
Jun 29, 2000·Annual Review of Biochemistry·M BorgniaP Agre
Feb 1, 1996·The Journal of Clinical Investigation·H G FolkessonA S Verkman
Oct 1, 1995·Kidney International·A S VerkmanT Ma
Jun 20, 2006·Biochimica Et Biophysica Acta·David Pitonzo, William R Skach
Aug 23, 2012·Molecular Membrane Biology·Fredrik Oberg, Kristina Hedfalk
Mar 15, 2013·Journal of Korean Medical Science·Hong Zhe ZhangHae-Rahn Bae
Jul 16, 2004·American Journal of Physiology. Cell Physiology·Teresa M BuckWilliam R Skach
Mar 9, 2002·The Journal of Biological Chemistry·Yoko Dohke, R James Turner
Feb 8, 2000·American Journal of Physiology. Renal Physiology·A S Verkman, A K Mitra
Nov 2, 2004·The Journal of Biological Chemistry·Teresa M Buck, William R Skach
May 8, 2018·RSC Advances·Jacopo MarinoOliver Zerbe
Jan 1, 1996·The American Journal of Physiology·A S VerkmanJ Farinas

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