PMID: 9430621Feb 28, 1998Paper

Distinct calcium channel isoforms mediate parathyroid hormone and chlorothiazide-stimulated calcium entry in transporting epithelial cells

The Journal of Membrane Biology
E L BarryP A Friedman

Abstract

Some cells express multiple calcium channel isoforms that are likely to have distinct functions. The present study used molecular cloning and antisense techniques to identify calcium channel isoforms mediating calcium entry in mouse distal convoluted tubule (DCT) cells. The DCT is the major site of hormone- and diuretic-regulated calcium transport in the kidney. Cellular calcium absorption involves entry through apical membrane calcium channels that are sensitive to dihydropyridine-type calcium channel antagonists. Partial cDNA clones corresponding to one isoform of the calcium channel alpha1 pore-forming subunit, alpha1C, and one isoform of the calcium channel beta accessory subunit, beta3, were isolated by RT-PCR. Full-length transcripts were detected by Northern blot analysis in immortalized DCT cells. Antisense oligonucleotides complementary to the alpha1C sequence inhibited the rise of intracellular calcium ([Ca2+]i) induced by the thiazide diuretic, chlorothiazide (CTZ), but not that induced by parathyroid hormone (PTH). However, antisense oligonucleotides complementary to the beta3 sequence inhibited both CTZ- and PTH-induced rises of [Ca2+]i. beta3 antisense oligonucleotides also inhibited the membrane hyperpolarization...Continue Reading

Citations

Jul 27, 2001·Fundamental & Clinical Pharmacology·L GalánJ L Alvarez
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Dec 14, 1999·Current Opinion in Nephrology and Hypertension·P A Friedman
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Feb 20, 2010·American Journal of Respiratory and Critical Care Medicine·Marilena Djata CabralLucette Pelletier
Sep 22, 2009·Canadian Journal of Physiology and Pharmacology·José F BernardoPeter A Friedman
Jul 14, 2004·Molecular and Cellular Endocrinology·Denis CouchourelMichèle G Brunette
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Mar 7, 2003·American Journal of Physiology. Renal Physiology·Johannes Loffing, Brigitte Kaissling
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