PMID: 8970162Dec 1, 1996Paper

Distinct cellular locations of the syntaxin family of proteins in rat pancreatic acinar cells

Molecular Biology of the Cell
Herbert Y GaisanoW S Trimble

Abstract

Syntaxins are cytoplasmically oriented integral membrane soluble NEM-sensitive factor receptors (SNAREs; soluble NEM-sensitive factor attachment protein receptors) thought to serve as targets for the assembly of protein complexes important in regulating membrane fusion. The SNARE hypothesis predicts that the fidelity of vesicle traffic is controlled in part by the correct recognition of vesicle SNAREs with their cognate target SNARE partner. Here, we show that in the exocrine acinar cell of the pancreas, multiple syntaxin isoforms are expressed and that they appear to reside in distinct membrane compartments. Syntaxin 2 is restricted to the apical plasma membrane whereas syntaxin 4 is found most abundantly on the basolateral membranes. Surprisingly, syntaxin 3 was found to be localized to a vesicular compartment, the zymogen granule membrane. In addition, we show that these proteins are capable of specific interaction with vesicle SNARE proteins. Their nonoverlapping locations support the general principle of the SNARE hypothesis and provide new insights into the mechanisms of polarized secretion in epithelial cells.

References

Mar 11, 1977·Virchows Archiv. A, Pathological Anatomy and Histology·M Lampel, H F Kern
Jan 1, 1989·The Journal of Clinical Investigation·H Y GaisanoL J Miller
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W S TrimbleR H Scheller
Jul 7, 1995·The Journal of Biological Chemistry·M TagayaS Mizushima
Sep 10, 1993·Cell·M K BennettR H Scheller
May 19, 1995·Cell·K L Wilson
Mar 1, 1995·The Journal of Cell Biology·K SadoulP A Halban
Jan 1, 1994·Annual Review of Biochemistry·M K Bennett, R H Scheller
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J PevsnerR H Scheller
Mar 8, 1996·The Journal of Biological Chemistry·J C HayR H Scheller

❮ Previous
Next ❯

Citations

Jul 14, 2001·Archives of Oral Biology·A ImaiH Shimomura
Jun 1, 1997·Current Opinion in Neurobiology·P I HansonR Jahn
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·Thomas WeimbsGeri Kreitzer
Sep 11, 2010·The Journal of Biological Chemistry·Michelle A FalkowskiGuy E Groblewski
Feb 1, 2011·The Journal of Biological Chemistry·Jacqueline TorresMartin B A ter Beest
Jun 16, 2001·Molecular Biology of the Cell·F WendlerS A Tooze
Dec 1, 1996·Molecular Biology of the Cell·S H LowK E Mostov
Jul 1, 1997·Molecular Biology of the Cell·J B BockR H Scheller
Oct 7, 2005·Molecular Biology of the Cell·Martin B A ter BeestKeith E Mostov
May 26, 1998·Annals of the New York Academy of Sciences·J D Castle
Jun 26, 2007·Journal of Bioinformatics and Computational Biology·Gregory PenningtonRussell Schwartz
Apr 1, 1999·Annual Review of Physiology·J AveryJ M Edwardson
Mar 14, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ning WengGuy E Groblewski
Jan 31, 1998·The Journal of Clinical Investigation·H OhnishiJ A Williams
Jun 7, 2008·The Journal of Clinical Investigation·Laura I Cosen-BinkerHerbert Y Gaisano
Jun 10, 2005·BMC Cell Biology·Arja M Band, Esa Kuismanen
Apr 18, 2008·World Journal of Gastroenterology : WJG·Laura-I Cosen-BinkerHerbert Y Gaisano
Jun 2, 2000·Biochemical and Biophysical Research Communications·R MinakamiH Sugiyama
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·T WeimbsK Hofmann
Oct 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sharmila BhattacharyaThomas L Schwarz
Apr 20, 2014·Cell Calcium·Scott W MessengerGuy E Groblewski
Oct 27, 2010·Expert Review of Proteomics·María Gómez-LázaroMichael Schrader
Jun 21, 2006·The Journal of Cell Biology·Nikunj SharmaThomas Weimbs
Dec 29, 1998·The Journal of Cell Biology·S UrbéS A Tooze
Feb 24, 2016·Frontiers in Molecular Neuroscience·Suleman HussainSvend Davanger
Sep 1, 1999·Kidney International·S LehtonenE Lehtonen
Mar 10, 2012·Biochimica Et Biophysica Acta·Peter Thorn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.