Distinct constrictive processes, separated in time and space, divide caulobacter inner and outer membranes

Journal of Bacteriology
Ellen M JuddHarley H McAdams


Cryoelectron microscope tomography (cryoEM) and a fluorescence loss in photobleaching (FLIP) assay were used to characterize progression of the terminal stages of Caulobacter crescentus cell division. Tomographic cryoEM images of the cell division site show separate constrictive processes closing first the inner membrane (IM) and then the outer membrane (OM) in a manner distinctly different from that of septum-forming bacteria. FLIP experiments had previously shown cytoplasmic compartmentalization (when cytoplasmic proteins can no longer diffuse between the two nascent progeny cell compartments) occurring 18 min before daughter cell separation in a 135-min cell cycle so the two constrictive processes are separated in both time and space. In the very latest stages of both IM and OM constriction, short membrane tether structures are observed. The smallest observed pre-fission tethers were 60 nm in diameter for both the inner and outer membranes. Here, we also used FLIP experiments to show that both membrane-bound and periplasmic fluorescent proteins diffuse freely through the FtsZ ring during most of the constriction procession.


Oct 1, 1977·Journal of Bacteriology·M Evinger, N Agabian
Jan 1, 1991·Methods in Enzymology·B Ely
Jul 1, 1981·Canadian Journal of Microbiology·J S Poindexter, J G Hagenzieker
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Jun 19, 1998·Molecular Microbiology·M J SackettYves V Brun
Dec 29, 1998·Journal of Bacteriology·M B ElowitzS Leibler
Jul 6, 2000·The EMBO Journal·Jeffrey M Skerker, Lucy Shapiro
May 15, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Thomas R PowersRaymond E Goldstein
Jun 8, 2002·Journal of Molecular Biology·Stephen G Addinall, Barry Holland
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert E CampbellRoger Y Tsien
Dec 10, 2002·Science·Lucy ShapiroR Losick
Jan 16, 2003·Current Biology : CB·William Margolin
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jeffery ErringtonDirk-Jan Scheffers
Mar 26, 2003·Annual Review of Biochemistry·Kathleen R Ryan, Lucy Shapiro
Jun 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ellen M JuddHarley H McAdams
Sep 27, 2003·Science·Harley H McAdams, Lucy Shapiro
Oct 7, 2003·Annual Review of Microbiology·Nora Ausmees, Christine Jacobs-Wagner
Apr 6, 2004·Current Opinion in Microbiology·Patrick T McGrathHarley H McAdams
Oct 20, 2004·Molecular Microbiology·David S Weiss
Nov 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·J DeichW E Moerner
Mar 9, 2005·Molecular Microbiology·Sebastien Pichoff, J Lutkenhaus


Jun 7, 2013·Journal of the American Chemical Society·Ye-Jin EunDouglas B Weibel
Aug 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiling ShenHarley H McAdams
Feb 8, 2013·Genes & Development·Thierry DoanDavid Z Rudner
Oct 4, 2005·Journal of Bacteriology·J Maddock
Mar 22, 2008·Annual Review of Biochemistry·Dylan M Morris, Grant J Jensen
Dec 14, 2007·Annual Review of Genetics·Michael T LaubHarley H McAdams
Jul 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Harley H McAdams
Jul 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jennifer K WagnerYves V Brun
Sep 22, 2009·FEBS Letters·Harley H McAdams, Lucy Shapiro
Mar 5, 2011·Journal of Molecular Biology·Harley H McAdams, Lucy Shapiro
Mar 1, 2016·Nature Reviews. Microbiology·Catherine M OikonomouYi-Wei Chang
Aug 22, 2007·Current Opinion in Biotechnology·Justine Collier, Lucy Shapiro
Sep 22, 2006·Molecular Microbiology·Terry J Beveridge
Sep 22, 2006·Molecular Microbiology·Ariane BriegelGrant J Jensen
May 6, 2011·Molecular Microbiology·Erin D GoleyLucy Shapiro
Jun 1, 2012·Molecular Microbiology·Dagmara Jakimowicz, Gilles P van Wezel
May 26, 2010·Molecular Microbiology·Erin D GoleyLucy Shapiro
Apr 5, 2016·Nature Reviews. Microbiology·Daniel P Haeusser, William Margolin
Sep 16, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Veronica W Rowlett, William Margolin
May 12, 2012·Current Biology : CB·An-Chun ChienP A Levin
Dec 15, 2010·Current Biology : CB·Tushar K Beuria, William Margolin
May 13, 2008·Journal of Structural Biology·Jacques IzardMichael Marko
Oct 29, 2014·PLoS Biology·W Seth ChildersLucy Shapiro
Mar 31, 2016·Molecular Microbiology·Elizabeth L MeierErin D Goley
Jul 13, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Harold P Erickson
Apr 28, 2016·Nature Communications·Thomas H MannLucy Shapiro
Oct 12, 2007·Physical Biology·Supratim Sengupta, Andrew Rutenberg
Oct 24, 2008·The ISME Journal·Luis R ComolliJillian F Banfield
Feb 25, 2020·Annual Review of Biophysics·Ryan McQuillen, Jie Xiao
Jan 10, 2018·Molecular Microbiology·Alexander J F Egan

Related Concepts

Outer Membrane Lipoproteins, Bacterial
Cell Compartmentation
Caulobacter crescentus
Cryoelectron Microscopy

Related Feeds