Distinct domains of Escherichia coli IgaA connect envelope stress sensing and down-regulation of the Rcs phosphorelay across subcellular compartments

PLoS Genetics
Nahla A HusseinJean-Francois Collet

Abstract

In enterobacteria, the Rcs system (Regulator of capsule synthesis) monitors envelope integrity and induces a stress response when damages occur in the outer membrane or in the peptidoglycan layer. Built around a two-component system, Rcs controls gene expression via a cascade of phosphoryl transfer reactions. Being particularly complex, Rcs also involves the outer membrane lipoprotein RcsF and the inner membrane essential protein IgaA (Intracellular growth attenuator). RcsF and IgaA, which are located upstream of the phosphorelay, are required for normal Rcs functioning. Here, we establish the stress-dependent formation of a complex between RcsF and the periplasmic domain of IgaA as the molecular signal triggering Rcs. Moreover, molecular dissection of IgaA reveals that its negative regulatory role on Rcs is mostly carried by its first N-terminal cytoplasmic domain. Altogether, our results support a model in which IgaA regulates Rcs activation by playing a direct role in the transfer of signals from the cell envelope to the cytoplasm. This remarkable feature further distinguishes Rcs from other envelope stress response systems.

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Citations

Jun 14, 2018·Annual Review of Microbiology·Erin WallSusan Gottesman
Jun 1, 2019·Nature Reviews. Microbiology·Angela M Mitchell, Thomas J Silhavy
Jun 4, 2020·The Journal of Biological Chemistry·Jean-François ColletCamille V Goemans
Dec 5, 2019·Frontiers in Cellular and Infection Microbiology·Antoine DelhayeGéraldine Laloux
May 22, 2019·Annual Review of Microbiology·Vanessa I Francis, Steven L Porter
Jun 24, 2020·Nature Chemical Biology·Raquel Rodríguez-AlonsoJean-François Collet
Oct 14, 2020·PLoS Biology·Vivek K MutalikAdam P Arkin
Sep 29, 2020·ELife·Kilian DekoninckJean-Francois Collet
Mar 6, 2021·Current Opinion in Microbiology·Jessica El RayesJean-François Collet
Feb 28, 2021·Nucleic Acids Research·Juanjo HuesaPatricia Casino
Mar 5, 2021·Frontiers in Microbiology·Jiao MengJingyu Chen
Mar 24, 2021·Journal of Bacteriology·Nickolas G KesslerSean D Moore
Jul 31, 2021·Nature Chemical Biology·Jessica El RayesJean-François Collet
Aug 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Muralidhar TataAnna Konovalova

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Methods Mentioned

BETA
environmental stress
immunoprecipitation
dissection
PCR
X-ray

Software Mentioned

TOPCONS
Prism
- Pad
Graph
IgaA

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