Distinct endotypes of steroid-resistant asthma characterized by IL-17A(high) and IFN-γ(high) immunophenotypes: Potential benefits of calcitriol

The Journal of Allergy and Clinical Immunology
Emma S ChambersC M Hawrylowicz

Abstract

A small population of patients with severe asthma does not respond to glucocorticoids (steroid resistant [SR]). They have high morbidity, highlighting an urgent need for strategies to enhance glucocorticoid responsiveness. We investigated the immunologic differences between steroid-sensitive (SS) and SR asthmatic patients and the effect on immunophenotype of oral calcitriol treatment because it has been previously shown to beneficially modulate the clinical response to glucocorticoids in patients with SR asthma. CD8-depleted PBMCs were isolated from 12 patients with SS and 23 patients with SR asthma and cultured for 7 days with anti-CD3 and IL-2 with or without dexamethasone. Cytokine production was assessed in supernatants by using the Cytometric Bead Array. Patients with SR asthma were subsequently randomized to oral calcitriol or placebo therapy, and identical studies were repeated. Patients with SR asthma produced significantly increased IL-17A and IFN-γ levels compared with those in patients with SS asthma, although it was evident that cells from individual patients might overproduce one or the other of these cytokines. Production of IL-17A was inversely and production of IL-13 was positively associated with the clinical r...Continue Reading

References

Jun 20, 2003·Respiratory Medicine·A BarczykE Sozañska
Nov 7, 2006·Respiratory Research·Dominique M A BullensJan L Ceuppens
Apr 18, 2008·Current Allergy and Asthma Reports·Ian M AdcockKian Fan Chung
Feb 4, 2009·The Journal of Clinical Investigation·David BendingAnne Cooke
Feb 12, 2009·Primary Care Respiratory Journal : Journal of the General Practice Airways Group·Mark L LevyPaul Stephenson
Apr 14, 2009·The Journal of Allergy and Clinical Immunology·Wisam Al-RamliQutayba Hamid
Jun 2, 2009·American Journal of Respiratory and Critical Care Medicine·Prescott G WoodruffJohn V Fahy
Feb 18, 2010·Expert Review of Clinical Immunology·Nancy E LangeScott Weiss
Mar 12, 2010·The American Journal of Clinical Nutrition·Mitsuyoshi UrashimaHiroyuki Ida
Mar 27, 2010·Respiratory Medicine·Ioana AgacheMariana Anghel
Jun 12, 2010·The Journal of Allergy and Clinical Immunology·John M BrehmUNKNOWN Childhood Asthma Management Program Research Group
Jun 16, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Vazquez-TelloQ Hamid
Sep 13, 2011·American Journal of Respiratory and Critical Care Medicine·Atul GuptaSejal Saglani
Feb 15, 2012·The Journal of Allergy and Clinical Immunology·Elena GolevaDonald Y M Leung
Jun 5, 2012·The Journal of Allergy and Clinical Immunology·Emma S ChambersCatherine M Hawrylowicz
Jul 17, 2012·American Journal of Respiratory and Critical Care Medicine·Ann Chen WuUNKNOWN Childhood Asthma Management Program Research Group
Aug 4, 2012·The Journal of Allergy and Clinical Immunology·Guiquan JiaUNKNOWN Bronchoscopic Exploratory Research Study of Biomarkers in Corticosteroid-refractory Asthma (BOBCAT) Study Group
May 22, 2013·The New England Journal of Medicine·Sally WenzelGianluca Pirozzi
Jul 23, 2013·The Journal of Allergy and Clinical Immunology·Michel LavioletteNestor A Molfino
Sep 18, 2013·Lancet·Fernando D Martinez, Donata Vercelli
Oct 1, 2013·The Journal of Allergy and Clinical Immunology·Michael C PetersJohn V Fahy
Nov 7, 2013·Indian Journal of Pediatrics·Madhu Yadav, Kundan Mittal
Dec 18, 2013·The European Respiratory Journal·Kian Fan ChungW Gerald Teague
Apr 18, 2014·Annals of the New York Academy of Sciences·Elizabeth H MannCatherine M Hawrylowicz
May 3, 2014·The Journal of Allergy and Clinical Immunology·Alexandra M NanzerCatherine M Hawrylowicz
May 20, 2014·JAMA : the Journal of the American Medical Association·Mario CastroUNKNOWN National Heart, Lung, and Blood Institute’s AsthmaNet
Aug 6, 2014·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Saba ArshiNadieh Akbarpour

❮ Previous
Next ❯

Citations

Mar 18, 2016·Journal of Research in Pharmacy Practice·Mohammad Esmaeil HejaziTaher Entezari-Maleki
Dec 19, 2015·Expert Review of Respiratory Medicine·Neil C Thomson
Jul 22, 2015·American Journal of Respiratory Cell and Molecular Biology·Nick C MatthewsTak H Lee
Mar 16, 2016·American Journal of Respiratory and Critical Care Medicine·John W Upham, Peter D Sly
Oct 25, 2016·Expert Opinion on Therapeutic Patents·Jason G KettleRichard Woessner
Feb 1, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Nathanael YatesCaitlin S Wyrwoll
Sep 1, 2016·American Journal of Respiratory and Critical Care Medicine·Chih-Hsi Scott KuoUNKNOWN U-BIOPRED Project Team ‡
Feb 18, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L Q C SiewC J Corrigan
Feb 19, 2016·European Journal of Immunology·Hye Young KimRosemarie H Dekruyff
Jan 17, 2017·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Kylie A MorganCatherine M Hawrylowicz
Mar 3, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D GençT Akkoç
Mar 10, 2018·Physiological Genomics·Charu RajputMarc B Hershenson
Sep 7, 2016·The Cochrane Database of Systematic Reviews·Adrian R MartineauChris J Griffiths
Nov 14, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E S ChambersC M Hawrylowicz
Oct 26, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Dasom KimIan P Lewkowich
Oct 26, 2018·American Journal of Rhinology & Allergy·Nuray Bayar MulukGuy Scadding
Jan 5, 2019·Current Opinion in Allergy and Clinical Immunology·Augusto A Litonjua
Nov 24, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Danica ProdanovicAlastair G Stewart
Nov 2, 2016·Current Opinion in Pulmonary Medicine·Manali MukherjeeParameswaran Nair
Sep 14, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shamus P KeelerMichael J Holtzman
Sep 19, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mitsuru NiwaTakafumi Suda
Aug 29, 2020·Reviews in Medical Virology·Majid Teymoori-Rad, Sayed Mahdi Marashi
May 4, 2017·American Journal of Respiratory Cell and Molecular Biology·Elizabeth H MannCatherine M Hawrylowicz
Aug 23, 2017·Expert Review of Respiratory Medicine·Aldara Martin AlonsoSejal Saglani
Oct 8, 2016·Respiratory Research·Thomas SouthworthDave Singh
Sep 2, 2017·American Journal of Respiratory Cell and Molecular Biology·Cameron H FlayerAngela Haczku
Aug 8, 2017·Science Immunology·Melody G DuvallUNKNOWN National Heart, Lung, and Blood Institute’s Severe Asthma Research Program-3 Investigators
Jan 29, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Jamille S FernandesLuciana S Cardoso
Jan 31, 2020·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Jermo Johannes van ToorGert Jan Braunstahl
Jan 2, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elizabeth H MannCatherine M Hawrylowicz
Feb 7, 2020·European Respiratory Review : an Official Journal of the European Respiratory Society·Arnaud BourdinPascal Chanez

❮ Previous
Next ❯

Methods Mentioned

BETA
bronchoalveolar lavage

Software Mentioned

GraphPad Prism
WinspiroPRO

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.