Distinct expression of the neurotoxic microRNA family let-7 in the cerebrospinal fluid of patients with Alzheimer's disease

PloS One
Katja DerkowSeija Lehnardt

Abstract

MicroRNAs (miRNAs) are non-coding RNAs originally involved in RNA silencing and post-transcriptional regulation of gene expression. We have shown in previous work that the miRNA let-7b can act as a signalling molecule for Toll-like receptor 7, thereby initiating innate immune pathways and apoptosis in the central nervous system. Here, we investigated whether different members of the miRNA family let-7, abundantly expressed in the brain, are released into the human cerebrospinal fluid (CSF) and whether quantitative differences in let-7 copies exist in neurodegenerative diseases. RNA isolated from CSF of patients with Alzheimer´s disease (AD) and from control patients with frontotemporal lobe dementia (FTLD), major depressive episode (MDE) without clinical or neurobiological signs of AD, and healthy individuals, was reverse transcribed with primers against nine let-7 family members, and miRNAs were quantified and analyzed comparatively by quantitative PCR. let-7 miRNAs were present in CSF from patients with AD, FTLD, MDE, and healthy controls. However, the amount of individual let-7 miRNAs in the CSF varied substantially. CSF from AD patients contained higher amounts of let-7b and let-7e compared to healthy controls, while no dif...Continue Reading

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Citations

Feb 6, 2019·Molecular Neurobiology·Verónica M Borgonio-CuadraJonathan J Magaña
Apr 6, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Verena Theis, Carsten Theiss
Aug 23, 2019·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Anna Niewiadomska-Cimicka, Yvon Trottier
Jun 18, 2020·Biomolecules·Maria Antonietta PanaroChiara Porro
Jul 10, 2019·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Sandra Van der AuweraHans J Grabe
Nov 17, 2019·Cancers·Michalina ZajdelJan Konrad Siwicki
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Sep 10, 2020·Journal of Personalized Medicine·Eva AusóGema Esquiva
Nov 11, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Efthalia AngelopoulouChristina Piperi
Feb 12, 2021·Molecular Neurobiology·Robert EppleAndre Fischer
Apr 4, 2021·International Journal of Molecular Sciences·Liya QinLeon G Coleman
Apr 2, 2021·Frontiers in Neuroscience·Athira AnirudhanShiek S S J Ahmed
May 3, 2021·Cellular and Molecular Neurobiology·Islauddin KhanShashi Bala Singh

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Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

GraphPad
GraphPad Prism
FlowJo

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