Distinct expression profiles of Notch-1 protein in human solid tumors: Implications for development of targeted therapeutic monoclonal antibodies.

Biologics : Targets & Therapy
Yuan LiZhi-Qiang Zhang

Abstract

Biological therapies, such as monoclonal antibodies (mAbs) that target tumor-associated antigens have been considered an effective therapeutic approach in oncology. In considering Notch-1 receptor as a potential target, we performed immunohistochemistry on tissue microarrays to determine 1) whether the receptor is overexpressed in tumor cells as compared to their corresponding normal tissues and 2) the clinical significance of its expression levels in human breast, colorectal, lung and prostate cancers. We found that the expression of Notch-1 protein was overexpressed in primary colorectal adenocarcinoma and nonsmall cell lung carcinoma (NSCLC), but not in primary ductal breast carcinoma or prostate adenocarcinoma. Further analysis revealed that higher levels of Notch-1 protein expression were significantly associated with poorer differentiation of breast and prostate tumors. Strikingly, for NSCLC, the expression levels of Notch-1 protein were found to be inversely correlated with tumor differentiation and progression. For colorectal tumors, however, no correlation of Notch-1 protein expression was found with any tumor clinicopathological parameters, in spite of its overexpression in tumor cells. Our data demonstrated the compl...Continue Reading

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Citations

May 2, 2012·Cancer Letters·Neil Ashley
Sep 6, 2015·Cancer Letters·Xun YuanKongming Wu
Sep 12, 2020·Molecular Pharmacology·Lobna Faiz GharaibehWalhan Alshaer

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Methods Mentioned

BETA
transfection
flow cytometry
electrophoresis

Software Mentioned

GraphPad Prism
GraphPad
DNA

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