Distinct GABAB actions via synaptic and extrasynaptic receptors in rat hippocampus in vitro

Journal of Neurophysiology
T M PhamJ C Lacaille

Abstract

Intracellular recordings were obtained from pyramidal cells to examine gamma-aminobutyric acid-B (GABAB)-mediated synaptic mechanisms in the CA1 region of rat hippocampal slices. To investigate if heterogeneous ionic mechanisms linked to GABAB receptors originate from distinct sets of inhibitory fibers, GABAB-mediated monosynaptic late inhibitory postsynaptic potentials (IPSPs) were elicited in the presence of antagonists of ionotropic glutamate and GABAA receptors and of an inhibitor of GABA uptake and were compared after direct stimulation of inhibitory fibers in three different CA1 layers: stratum oriens, radiatum, and lacunosum-moleculare. No significant differences were found in mean amplitude, rise time, or time to decay to half-amplitude of IPSPs evoked from the three layers. Mean equilibrium potential (Erev) of late IPSPs was similar for all groups and close to the equilibrium potential of K+. Bath application of the GABAB antagonist CGP55845A blocked all monosynaptic late IPSPs. During recordings with micropipettes containing guanosine-5'-O-(3-thiotriphosphate) (GTPgammaS), the mean amplitude of all GABAB IPSPs gradually was reduced. Bath application of Ba2+ completely eliminated monosynaptic late IPSPs evoked from any...Continue Reading

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Citations

Mar 17, 1999·The Journal of Comparative Neurology·M Margeta-MitrovicA I Basbaum
Dec 24, 2004·The Journal of Pharmacology and Experimental Therapeutics·Peter H WuWilliam R Proctor
Nov 17, 2017·Brain Structure & Function·Yaneri A Ayala, Manuel S Malmierca
Feb 13, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Fatima A NasrallahCaroline Rae
Nov 10, 2006·Journal of Neurophysiology·Tyler K BestZygmunt Galdzicki

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