Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration

Brain and Behavior
Xuan LiF Javier Rubio

Abstract

Methamphetamine (Meth) seeking progressively increases after withdrawal (incubation of Meth craving). We previously demonstrated a role of anterior intralaminar nucleus of thalamus (AIT) to dorsomedial striatum (DMS) projections in this incubation. Here, we examined molecular alterations in DMS and AIT neurons activated (identified by neuronal activity marker Fos) during "incubated" Meth-seeking relapse test after prolonged withdrawal. We trained male rats to self-administer Meth or saline (control condition) for 10 days (6 hr/day). Using fluorescence-activated cell sorting, we examined gene expression in Fos-positive (activated during a 2-hr relapse test) and Fos-negative (nonactivated) DMS and AIT neurons. In DMS, we found increased mRNA expressions of immediate early genes (IEGs) (Arc, Egr1, Npas4, Fosb), Trkb, glutamate receptors subunits (Gria3, Grin1, Grin2b, Grm1), and epigenetic enzymes (Hdac3, Hdac5, Crebbp) in Fos-positive neurons, compared with Fos-negative neurons. In AIT, we found that fewer genes (Egr1, Fosb, TrkB, Grin1, and Hdac5) exhibited increased mRNA expression in Fos-positive neurons. Unexpectedly, in both brain regions, gene alterations described above also occurred in drug-naïve saline self-administratio...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B HopeE J Nestler
Feb 1, 1994·Trends in Neurosciences·H J Groenewegen, H W Berendse
Mar 1, 2002·Pharmacological Reviews·Uri ShalevYavin Shaham
Apr 19, 2006·Journal of Cellular Biochemistry·Maude Tessier, James R Woodgett
Jul 2, 2008·Annals of the New York Academy of Sciences·Nicholas D Schiff
Jul 22, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Hans S CrombagYavin Shaham
Dec 2, 2008·Substance Abuse : Official Publication of the Association for Medical Education and Research in Substance Abuse·Ahmed ElkashefJari Tiihonen
Mar 18, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Danielle Guez-BarberBruce T Hope
Jul 19, 2011·Trends in Neurosciences·Charles L PickensYavin Shaham
Apr 12, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jennifer M BossertYavin Shaham
Oct 4, 2013·Nature Reviews. Neuroscience·Fabio C CruzBruce T Hope
Dec 3, 2014·Neuroscience and Biobehavioral Reviews·Anne Pereira de Vasconcelos, Jean-Christophe Cassel
Dec 6, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Xuan LiYavin Shaham
Apr 10, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·F Javier RubioBruce T Hope
Mar 24, 2016·British Journal of Cancer·J M SchuurhuisF K L Spijkervet
Jun 7, 2016·Biological Psychiatry·Andrew F ScheyerMarina E Wolf
Jun 24, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Brandon L WarrenBruce T Hope
Jan 27, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniele CaprioliYavin Shaham
May 26, 2017·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Joseph J ZiminskiEisuke Koya
Jan 27, 2018·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xuan LiYavin Shaham
Aug 4, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Hannah M CatesEric J Nestler
Oct 8, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·David J ReinerYavin Shaham

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
FCS
Assay
PCR
dissection
current
transgenic

Software Mentioned

Prism GraphPad
FACS
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.