Distinct gene expression profiles in different B-cell compartments in human peripheral lymphoid organs

BMC Immunology
Yulei ShenWing C Chan

Abstract

There are three major B-cell compartments in peripheral lymphoid organs: the germinal center (GC), the mantle zone (MNZ) and the marginal zone (MGZ). Unique sets of B-cells reside in these compartments, and they have specific functional roles in humoral immune response. MNZ B cells are naive cells in a quiescent state and may participate in GC reactions upon proper stimulation. The adult splenic MGZ contains mostly memory B cells and is also known to provide a rapid response to particulate antigens. The GC B-cells proliferate rapidly and undergo selection and affinity maturation. The B-cell maturational process is accompanied by changes in the expression of cell-surface and intracellular proteins and requires signals from the specialized microenvironments. We performed laser microdissection of the three compartments for gene expression profiling by cDNA microarray. The transcriptional program of the GC was dominated by upregulation of genes associated with proliferation and DNA repair or recombination. The MNZ and MGZ showed increased expression of genes promoting cellular quiescence. The three compartments also revealed distinct repertoires of apoptosis-associated genes, chemokines and chemokine receptors. The MNZ and GC showe...Continue Reading

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Methods Mentioned

BETA
laser
fluorescence-activated cell sorting
dissection
PCR
electrophoresis
FACS
in vitro transcription

Software Mentioned

MATLAB
Gene
TreeView
statistics
Tree View
SAM
Significance Analysis of Microarrays ( SAM )
OMIM

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