Distinct gene expression profiles in immortalized human urothelial cells exposed to inorganic arsenite and its methylated trivalent metabolites

Environmental Health Perspectives
Pei-Fen SuTe-Chang Lee

Abstract

Inorganic arsenic is an environmental carcinogen. The generation of toxic trivalent methylated metabolites complicates the study of arsenic-mediated carcinogenesis. This study systematically evaluated the effect of chronic treatment with sodium arsenite (iAs(III)), monomethylarsonous acid (MMA(III)), and dimethylarsinous acid (DMA(III)) on immortalized human uroepithelial cells (SV-HUC-1 cells) using cDNA microarray. After exposure for 25 passages to iAs(III) (0.5 microM), MMA(III) (0.05, 0.1, or 0.2 microM), or DMA(III) (0.2 or 0.5 microM), significant compound-specific morphologic changes were observed. A set of 114 genes (5.7% of the examined genes) was differentially expressed in one or more sets of arsenical-treated cells compared with untreated controls. Expression analysis showed that exposure of cells to DMA(III) resulted in a gene profile different from that in cells exposed to iAs(III) or MMA(III), and that the iAs(III)-induced gene profile was closest to that in the tumorigenic HUC-1-derived 3-methylcholanthrene-induced tumorigenic cell line MC-SV-HUC T2, which was derived from SV-HUC-1 cells by methylcholanthrene treatment. Of the genes affected by all three arsenicals, only one, that coding for interleukin-1 recept...Continue Reading

References

Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·C Q ZhaoM P Waalkes
Aug 26, 2000·Cytokine & Growth Factor Reviews·R Iwamoto, E Mekada
Mar 23, 2001·Nature Immunology·S K Dower
Mar 13, 2001·Seminars in Cancer Biology·J M Pipas, A J Levine
May 16, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·J LiuM P Waalkes
Sep 7, 2001·Cytokine & Growth Factor Reviews·K Xie
Oct 17, 2001·Toxicology and Applied Pharmacology·D J ThomasS Lin
Feb 12, 2002·Environmental Health Perspectives·Jie LiuMichael P Waalkes
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Keiko FujikawaWojciech Swat
Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Aug 22, 2002·Nature Reviews. Cancer·Jean Paul Thiery
Sep 27, 2002·Toxicology·Jiro TakataYoshiharu Karube
Oct 16, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hisham K HamadehDori Germolec
Nov 12, 2002·Environmental Health Perspectives·Miroslav StýbloDavid J Thomas
Dec 19, 2002·Journal of the National Cancer Institute·William E AchanzarMichael P Waalkes
Dec 19, 2002·Environmental and Molecular Mutagenesis·J A BarnesJ W Allen
Mar 15, 2003·Nature Reviews. Cancer·John D Hood, David A Cheresh
Apr 8, 2003·Toxicology·Xing Hui ZhengA Jay Gandolfi
May 2, 2003·Annals of the New York Academy of Sciences·Jessica E Sutherland, Max Costa
May 3, 2003·Carcinogenesis·Miguel A ReaRobert H Rice
May 30, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Dong-Soon BaeJulie A Campain
Jun 13, 2003·Environmental and Molecular Mutagenesis·Kanae MureToby G Rossman
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·H Weiler, B H Isermann
Jul 31, 2003·Journal of Inorganic Biochemistry·Yong QianXianglin Shi
Aug 21, 2003·Environmental Health Perspectives·Meei-Maan WuTe-Chang Lee

❮ Previous
Next ❯

Citations

Apr 24, 2008·Metal-based Drugs·Stephen John Ralph
Apr 5, 2011·Biomarker Insights·Udensi K UdensiRaphael D Isokpehi
Jun 15, 2007·Journal of Toxicology and Environmental Health. Part a·James W DuMond, Kamaleshwar P Singh
Oct 20, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Horng-Rong ChangChau-Jong Wang
Mar 6, 2007·Nihon eiseigaku zasshi. Japanese journal of hygiene·Hidekuni InaderaAkiko Shimomura
Jun 25, 2009·Environmental and Molecular Mutagenesis·P Robinan GentryHarvey J Clewell
Nov 14, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Keiko NoharaTakahiro Kobayashi
Apr 18, 2013·Environmental Science & Technology·Hiroe Hara-YamamuraSatoshi Okabe
Oct 11, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jara J BruntJohn J Heikkila

❮ Previous
Next ❯

Methods Mentioned

BETA
chips
PCR
electrophoresis
Protein Assay
transgenic
environmental stress

Software Mentioned

GenePix
CalcuSyn

Related Concepts

Related Feeds

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Related Papers

Mutation Research
Soile Tapio, Bernd Grosche
Toxicological Sciences : an Official Journal of the Society of Toxicology
Hisham K HamadehD R Germolec
Toxicological Sciences : an Official Journal of the Society of Toxicology
Donald A SensSeema Somji
© 2021 Meta ULC. All rights reserved