Distinct genotypes of human and canine isolates of Campylobacter upsaliensis determined by 16S rRNA gene typing and plasmid profiling.

Journal of Clinical Microbiology
J StanleyR J Owen

Abstract

The utility of combined 16S rRNA (rrs) gene restriction fragment length polymorphism and plasmid profiles to differentiate between and within Campylobacter upsaliensis of human and canine origin was examined. Fourteen distinct rrs gene restriction fragment length polymorphs consisting of bands sized between 1.9 and 4.8 kb were observed. The copy number of the 16S rRNA gene was three in most strains of C. upsaliensis. Plasmids were found in almost 60% of the strains; ranging in size from 1.5 to 100 kb, they gave 15 distinct plasmid profiles. All isolates from humans contained one or more plasmids, as did strains isolated from dogs with sporadic diarrhea. The two commonest 16S ribotypes were divided into eight and nine subgroups by plasmid profiling. The genotyping of canine isolates from three veterinary surveys detected both multiple infections and reinfection of dogs. Except for one, each of the isolates from humans constituted a single and unique 16S ribotype, and these more frequently carried plasmids than did canine strains. Ribotypes of human strains were not found among canine isolates. These results suggest that host-specific genotypic differences may exist among strains of C. upsaliensis, for example, intraspecific clon...Continue Reading

References

Apr 1, 1992·Journal of Bacteriology·D E TaylorN Chang
Mar 1, 1990·Bio/technology·T BarryF Gannon
Dec 1, 1992·The Journal of Applied Bacteriology·D LintonJ Stanley
Jul 1, 1992·Journal of Medical Microbiology·F M Da Silva TatleyL M Steyn
Apr 1, 1991·Journal of Clinical Microbiology·C M PattonH Lior
May 1, 1990·Journal of Clinical Microbiology·H GoossensJ Verhoef
Jul 1, 1987·Journal of Clinical Microbiology·W TeeB Dwyer
Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Feb 1, 1993·Molecular and Cellular Probes·E EzquerraJ Stanley

❮ Previous
Next ❯

Citations

Jan 5, 2000·Applied and Environmental Microbiology·T M Wassenaar, D G Newell
May 17, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jaime A LabarcaLaurene Mascola
Nov 11, 2003·Scandinavian Journal of Infectious Diseases·Eva Olsson EngvallLena Englund
Jul 17, 1998·Clinical Microbiology Reviews·B BourkeP Sherman
Feb 1, 1996·Journal of Clinical Microbiology·C FitzgeraldJ Stanley
Apr 1, 1995·Journal of Clinical Microbiology·R J OwenJ Stanley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Campylobacteriosis

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.