Distinct HIV-1 long terminal repeat quasispecies present in nervous tissues compared to that in lung, blood and lymphoid tissues of an AIDS patient

AIDS
M Ait-KhaledV C Emery

Abstract

To investigate the phylogenetic relationship of HIV-1 proviral long terminal repeat (LTR) variants present in postmortem samples of lymph node, spleen, lung, dorsal root ganglion and spinal cord as well as in the peripheral blood of an HIV-1-infected patient dying with AIDS. Postmortem tissues were studied by a combination of histology, cell culture and molecular analyses. The patient had a stable CD4 count of 10 x 10(6)/I during the 12 months preceding death. A 540 base-pair fragment of the LTR including U3/R/U5 was amplified using polymerase chain reaction on proviral DNA from the five postmortem tissues and peripheral blood mononuclear cells obtained 2 months prior to death. The population of viral variants was determined by sequencing at least five plasmid clones of the amplicons. The relationship between the variants present in different body sites was investigated using molecular phylogeny methods. HIV-1 was present in all organs analysed and correlated with the presence of abnormal histology. Genetic variation leading to divergence from the consensus sequence was more frequently present in characterized transcription factor binding sites within the LTR (P < 0.0001) although the HIV-1 LTR quasispecies in the different bod...Continue Reading

Citations

Jun 25, 2002·AIDS Research and Human Retroviruses·Caterina SerraAntonina Dolei
Jun 25, 1999·AIDS Research and Human Retroviruses·P ShapshakK Goodkin
Aug 29, 2012·AIDS Research and Human Retroviruses·Lachlan R GrayMelissa J Churchill
Oct 18, 2002·Antimicrobial Agents and Chemotherapy·Marta BoffitoGiovanni Di Perri
Oct 29, 2003·Journal of Virology·Prasith BaccamSusan Carpenter
Jul 1, 2011·Future Virology·Maile Ay Karris, Davey M Smith
Jun 4, 1998·Journal of Neuroimmunology·K GoodkinA M Kumar
Sep 1, 1996·Infection·A LafeuilladeC Sayada
Jan 6, 2016·Journal of Neurovirology·Lachlan R GrayMelissa J Churchill
Jul 26, 2014·Journal of Neurovirology·Melissa J ChurchillLachlan R Gray
May 18, 1999·Biochemical and Biophysical Research Communications·M RadkowskiT Laskus
Oct 18, 2016·Frontiers in Immunology·Céline MarbanChristian Schwartz
Dec 19, 2017·Virus Evolution·Christopher J R IllingworthJudith Breuer
Jun 26, 2004·Journal of Leukocyte Biology·Simon J PotterNitin K Saksena
Sep 10, 2003·Journal of Leukocyte Biology·Olivier RohrEvelyne Schaeffer
Mar 5, 2020·Reviews in Medical Virology·Jason T BlackardBenjamin L Laskin
Jan 2, 2021·Pathogens·Aneta PlutaCésar N Cortés-Rubio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.