Distinct human stem cell populations in small and large intestine

PloS One
Julie M CramerEric Lagasse

Abstract

The intestine is composed of an epithelial layer containing rapidly proliferating cells that mature into two regions, the small and the large intestine. Although previous studies have identified stem cells as the cell-of-origin for intestinal epithelial cells, no studies have directly compared stem cells derived from these anatomically distinct regions. Here, we examine intrinsic differences between primary epithelial cells isolated from human fetal small and large intestine, after in vitro expansion, using the Wnt agonist R-spondin 2. We utilized flow cytometry, fluorescence-activated cell sorting, gene expression analysis and a three-dimensional in vitro differentiation assay to characterize their stem cell properties. We identified stem cell markers that separate subpopulations of colony-forming cells in the small and large intestine and revealed important differences in differentiation, proliferation and disease pathways using gene expression analysis. Single cells from small and large intestine cultures formed organoids that reflect the distinct cellular hierarchy found in vivo and respond differently to identical exogenous cues. Our characterization identified numerous differences between small and large intestine epithel...Continue Reading

References

Sep 17, 1992·Nature·S M PowellK W Kinzler
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S HeimfeldD Rennick
Mar 1, 1984·The Journal of Cell Biology·U K EhmannD S Misfeldt
Mar 31, 1999·Immunology Today·D RiemannJ Langner
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·N UchidaI L Weissman
Dec 18, 2001·Cytometry·R P WerstoE Gabrielson
May 12, 2004·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·M QuinteroP A Brennan
Jun 29, 2004·International Review of Cytology·Rolf Mentlein
Jun 1, 2005·Experimental Cell Research·Daniel Pinto, Hans Clevers
Jun 14, 2005·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·Ph Meijnen, E J Th Rutgers
Jun 17, 2005·Nature·Silvia FreSpyros Artavanis-Tsakonas
Apr 25, 2006·Carcinogenesis·Carlos H F ChanClifford P Stanners
Nov 23, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Oleg K GlebovIlan R Kirsch
Nov 24, 2006·Nature·Lucia Ricci-VitianiRuggero De Maria
Mar 8, 2008·Gastroenterology·David H ScovilleLinheng Li
Sep 2, 2008·Cancer Research·Christine OdouxEric Lagasse
Dec 19, 2008·Nature·Nick BarkerHans Clevers
Feb 27, 2009·PLoS Biology·Wouter BossuytBassem A Hassan
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Silvia FreDaniel Louvard
Jan 1, 2008·Cold Spring Harbor Symposia on Quantitative Biology·N BarkerH Clevers
Sep 19, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Wang LikuiZhang Shuwen
Dec 25, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Richard J von FurstenbergSusan J Henning
Jun 17, 2011·Nature·Jan Paul Medema, Louis Vermeulen
Sep 6, 2011·Nature Medicine·Peter JungEduard Batlle
Oct 8, 2011·Current Colorectal Cancer Reports·Avedis Kazanjian, Noah F Shroyer
May 24, 2012·The EMBO Journal·Jurian Schuijers, Hans Clevers
Nov 1, 2012·PloS One·Jianjin HuangLing Ding

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Citations

Feb 9, 2016·Developmental and Comparative Immunology·Alessandro BonfiniNicolas Buchon
Dec 25, 2015·Current Problems in Surgery·Joseph F PierreKenneth A Kudsk
Sep 15, 2017·Stem Cells International·Bailey C E PeckPraveen Sethupathy
Apr 25, 2019·International Journal of Molecular Sciences·Maria Giovanna FrancipaneEric Lagasse
Aug 23, 2019·World Journal of Gastroenterology : WJG·Jun-Hwan Yoo, Mark Donowitz
Oct 6, 2017·Current Pathobiology Reports·Maria Giovanna Francipane, Eric Lagasse
Dec 21, 2020·Journal of Crohn's & Colitis·Martina PolettiTamas Korcsmaros
Feb 13, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Pan XuDaisy Jonkers
Mar 9, 2021·Frontiers in Pediatrics·Lauren O'ConnellCarol M Aherne
May 15, 2021·Stem Cell Research & Therapy·Panida SittipoYun Kyung Lee
Jul 24, 2021·Alimentary Pharmacology & Therapeutics·Nicolas PierreEdouard Louis
Jun 9, 2021·Molecular Cancer Research : MCR·Allison E BridgesMuthusamy Thangaraju
Oct 7, 2021·Physiological Genomics·Zachary K CrissNoah F Shroyer

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Datasets Mentioned

BETA
GSE56525

Methods Mentioned

BETA
Fluorescence-Activated Cell Sorting
PCR
gene array
gene
flow cytometry

Software Mentioned

FlowJo
Ingenuity Pathway Analysis Software
Ingenuity Pathways Analysis

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