Distinct isoforms of protein kinase C are involved in human eosinophil functions induced by platelet-activating factor

International Archives of Allergy and Immunology
Takumi TakizawaA Morikawa

Abstract

Platelet-activating factor (PAF) is a potent stimulator of eosinophils. Recently, treatment with a protein kinase C (PKC) inhibitor which generally inhibits PKC isoforms has been shown to modulate several eosinophil functions in distinct manners, in that PKC inhibition enhanced CD11b expression and cellular adhesion, but inhibited superoxide generation and degranulation in PAF-stimulated human eosinophils. These results suggested that distinct PKC isoforms were likely to be involved in each eosinophil function induced by PAF. We have therefore investigated whether or not the PKC isoforms involved in PAF-induced CD11b expression and superoxide generation were different. Human eosinophils prepared from healthy volunteers were treated with PKC inhibitors, bis-indolylmaleimide I (BisI; a general PKC inhibitor), myristoylated PKC inhibitor peptide (myr-psiPKC; a PKCalpha, beta and delta inhibitor) and rottlerin (a PKCdelta inhibitor), followed by stimulation with PAF. CD11b expression was determined using flow cytometry and superoxide generation was evaluated using a cytochrome c reduction assay. BisI treatment led to enhancement of PAF-induced CD11b expression, while myr-psiPKC and rottlerin did not. In contrast, PAF-induced supero...Continue Reading

Citations

Jun 11, 2005·International Archives of Allergy and Immunology·Masahiko KatoHirokazu Kimura
Jul 4, 2006·European Journal of Pharmacology·Charles I Ezeamuzie, Najla Taslim
Apr 29, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Konrad PazdrakAlexander Kurosky
Oct 27, 2004·British Journal of Pharmacology·Charles I Ezeamuzie, Najla Taslim
Apr 28, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kimberly D DyerHelene F Rosenberg

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