Distinct K+ conductive pathways are required for Cl- and K+ secretion across distal colonic epithelium

American Journal of Physiology. Cell Physiology
Susan T HalmDan R Halm

Abstract

Secretion of Cl(-) and K(+) in the colonic epithelium operates through a cellular mechanism requiring K(+) channels in the basolateral and apical membranes. Transepithelial current [short-circuit current (I(sc))] and conductance (G(t)) were measured for isolated distal colonic mucosa during secretory activation by epinephrine (Epi) or PGE(2) and synergistically by PGE(2) and carbachol (PGE(2) + CCh). TRAM-34 at 0.5 microM, an inhibitor of K(Ca)3.1 (IK, Kcnn4) K(+) channels (H. Wulff, M. J. Miller, W. Hänsel, S. Grissmer, M. D. Cahalan, and K. G. Chandy. Proc Natl Acad Sci USA 97: 8151-8156, 2000), did not alter secretory I(sc) or G(t) in guinea pig or rat colon. The presence of K(Ca)3.1 in the mucosa was confirmed by immunoblot and immunofluorescence detection. At 100 microM, TRAM-34 inhibited I(sc) and G(t) activated by Epi ( approximately 4%), PGE(2) ( approximately 30%) and PGE(2) + CCh ( approximately 60%). The IC(50) of 4.0 microM implicated involvement of K(+) channels other than K(Ca)3.1. The secretory responses augmented by the K(+) channel opener 1-EBIO were inhibited only at a high concentration of TRAM-34, suggesting further that K(Ca)3.1 was not involved. Sensitivity of the synergistic response (PGE(2) + CCh) to a h...Continue Reading

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May 7, 2010·American Journal of Physiology. Cell Physiology·Christian BarmeyerVazhaikkurichi M Rajendran
Jun 1, 2012·American Journal of Physiology. Cell Physiology·Geoffrey I Sandle, Vazhaikkurichi M Rajendran
Feb 11, 2012·American Journal of Physiology. Cell Physiology·Satish K SinghVazhaikkurichi M Rajendran
Apr 24, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Susan T HalmDan R Halm
May 23, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jin ZhangDan R Halm
Jun 6, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jin ZhangDan R Halm
Jul 10, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·N S Nanda KumarVazhaikkurichi M Rajendran
Oct 16, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jin ZhangDan R Halm
Aug 27, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kanthesh M BasalingappaWilliam F Wonderlin
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Sep 1, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jonathan M NicholsAldebaran M Hofer
Oct 6, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Poonam J BajwaChristian Lytle
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