Distinct localization of GLUT-1, -3, and -5 in human monocyte-derived macrophages: effects of cell activation

The American Journal of Physiology
D MalideI A Simpson

Abstract

We determined subcellular localization of GLUT-1, GLUT-3, and GLUT-5 as human monocytes differentiate into macrophages in culture, and effects of the activating agents N-formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol myristate acetate (PMA). Western blot analysis demonstrated progressively increased GLUT-1, rapidly decreased GLUT-3, and a delayed increase of GLUT-5 expression during differentiation. Confocal microscopy revealed that each isoform displayed a unique subcellular distribution and cell-activation response. GLUT-1 was localized primarily to the cell surface but was also detected in the perinuclear region in a pattern characteristic of recycling endosomes. GLUT-3 exhibited predominantly a distinct vesicle-like staining but was present only in monocytes. GLUT-5 was found primarily at the cell surface but was detectable intracellularly. Activation with fMLP induced similar GLUT-1 and GLUT-5 redistributions from intracellular compartments toward the cell surface. PMA elicited a similar translocation of GLUT-1, but GLUT-5 was redistributed from the plasma membrane to a distinct intracellular compartment that appeared connected to the cell surface. These results suggest specific subcellular targeting of each tran...Continue Reading

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Citations

Jun 26, 2008·American Journal of Physiology. Endocrinology and Metabolism·Ian A SimpsonSusan J Vannucci
Apr 10, 2008·American Journal of Physiology. Endocrinology and Metabolism·Veronique Douard, Ronaldo P Ferraris
Nov 26, 2009·AJR. American Journal of Roentgenology·Vladimir SopovDavid Groshar
Jan 29, 2016·Gastroenterology Research and Practice·Matthew P MoyMichael S Gee
Sep 10, 2019·Frontiers in Immunology·Omar SharifGernot Schabbauer
Jun 15, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Philip C CalderPhilip Newsholme
Nov 24, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Daniel P SchusterMichael Mueckler
Nov 27, 2020·Frontiers in Immunology·John V ForresterMirela Delibegovic
Jul 19, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Rishi SharmaRangaramanujam M Kannan

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