PMID: 9420271Jan 7, 1998Paper

Distinct mechanisms trigger apoptosis in human immunodeficiency virus type 1-infected and in uninfected bystander T lymphocytes

Journal of Virology
G HerbeinE Verdin

Abstract

Apoptosis is a main feature of AIDS pathogenesis and is thought to play a role in the progressive decrease of CD4+ T lymphocytes in infected individuals. To determine whether apoptosis occurs in infected and/or in uninfected peripheral blood T lymphocytes, we have used a recombinant human immunodeficiency virus type 1 (HIV-1) infectious clone expressing the green fluorescent protein (GFP). Using flow cytometry, we have determined the incidence of apoptosis by either terminal transferase dUTP nick end labeling or annexin-V assays in different cell subpopulations, i.e., in CD4+ or CD8+ T cells that were GFP positive or negative. After HIV-1 infection of purified peripheral blood lymphocytes, we observed that apoptosis occurred mostly in infected CD4+ peripheral blood lymphocytes. Remarkably, the presence of monocyte-derived macrophages in the culture increased dramatically the apoptosis of uninfected bystander T lymphocytes, while apoptosis in HIV-infected T lymphocytes was not changed. We therefore demonstrate that HIV-induced apoptosis results from at least two distinct mechanisms: (i) direct apoptosis in HIV-infected CD4+ T lymphocytes and (ii) indirect apoptosis in uninfected T cells mediated by antigen-presenting cells.

References

Jul 10, 1992·Science·L MeyaardF Miedema
Oct 1, 1992·The Journal of Experimental Medicine·N K BandaT H Finkel
Apr 1, 1991·Immunology Today·J C Ameisen, A Capron
Dec 1, 1991·Virology·A G Laurent-CrawfordA G Hovanessian
Feb 15, 1991·Science·D E MosierS A Spector
May 1, 1991·The Journal of Clinical Investigation·C TeraiD A Carson
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B YoffeF B Hollinger
Jan 1, 1985·Immunogenetics·R D SalterP Cresswell
Aug 5, 1993·Journal of Molecular Biology·M F LaspiaM B Mathews
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M X WuS F Schlossman
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·K SakselaD Baltimore
Nov 1, 1994·Clinical and Experimental Immunology·A C JalecoR M Victorino
Jun 24, 1993·Nature·M L BonyhadiH Kaneshima
Jun 1, 1993·AIDS Research and Human Retroviruses·M L GougeonL Montagnier
Jan 1, 1993·Research in Virology·M CollinS Gordon
Jun 24, 1993·Nature·G M AldrovandiJ A Zack

❮ Previous
Next ❯

Citations

Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·C CicalaA S Fauci
Mar 27, 2002·Journal of Biomedical Science·Mingjie ZhangAndrew I Dayton
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melissa K CallahanAndrew J Henderson
May 21, 2005·Cell Death and Differentiation·J S LadhaD Mitra
Sep 23, 2009·Apoptosis : an International Journal on Programmed Cell Death·Manoj Kumar Tripathy, Debashis Mitra
Oct 22, 2015·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·UNKNOWN Mitteilungen des Arbeitskreises Blut des Bundesministeriums für Gesundheit
Mar 17, 2000·Proceedings of the Society for Experimental Biology and Medicine·G Herbein, W A O'Brien
Apr 16, 2005·Cell Death and Differentiation·M-L Gougeon
Apr 9, 2005·Cell Death and Differentiation·B HurtrelJ Estaquier
Jun 10, 2004·AIDS Research and Human Retroviruses·Ana MachucaIndira Hewlett
Jun 16, 2004·Clinical and Experimental Immunology·S SalmenL Berrueta
May 27, 2003·Nature Reviews. Immunology·Marie-Lise Gougeon
Apr 9, 2005·Cell Death and Differentiation·C PetrovasP D Katsikis
Dec 17, 2004·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Jun ZhengShen Pang
Jul 24, 2008·Journal of Medical Virology·Davide GibelliniMaria Carla Re
Nov 8, 2018·Journal of Neurovirology·Dorota Piekna-PrzybylskaSanjay B Maggirwar
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·U MahlknechtG Herbein
Mar 23, 2002·The Journal of Biological Chemistry·Yili YangJonathan D Ashwell
May 29, 2004·The Journal of General Virology·Anne-Zélie DecrionGeorges Herbein
May 15, 2004·DNA and Cell Biology·Parithosh K TungaturthiAlagarsamy Srinivasan
Sep 30, 2009·Expert Opinion on Drug Metabolism & Toxicology·David James Hooker, Catherine Louise Cherry
Apr 2, 1999·Immunology and Cell Biology·A Jaworowski, S M Crowe
Jul 8, 2005·Journal of Leukocyte Biology·Amanda BrownCecilia Cheng-Mayer
Feb 24, 2004·Journal of Cellular Physiology·Luca BenettiCristina Parolin
Apr 12, 2003·AIDS Research and Human Retroviruses·Jean-Charles GrivelLeonid B Margolis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis