Distinct mechanosensitive Ca2+ influx mechanisms in human primary synovial fibroblasts

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Yuko SakamotoEri Arikawa-Hirasawa

Abstract

Synovial cells are exposed to continually changing dynamic forces and are implicated in the maintenance of joint homeostasis. However, the mechanisms of synovial cell responses to mechanical stress are unclear. In this study, we investigated the difference between the mechanosensitive channels of human primary synovial fibroblasts (SFBs) and human primary dermal fibroblasts (DFBs) in response to mechanical stretch by uniaxial cyclic stretching and mechanical cell membrane deformation in vitro. Cyclic stretching induced orientation of SFBs and DFBs perpendicularly to the stretching direction. Furthermore, uniaxial stretching increased intracellular Ca(2+) levels in both cell types. The perpendicular orientation of DFBs was blocked by gadolinium (III) chloride (Gd(3+), a mechanosensitive Ca(2+) channel blocker) or ruthenium red (RR, a nonselective Ca(2+) channel blocker). However, Gd(3+) did not block the stretch-induced perpendicular orientation in SFBs, while RR inhibited this orientation. Similarly, Ca(2+) influx in DFBs induced by uniaxial stretching and membrane deformation was inhibited by Gd(3+), RR, and GsMTx-4 (another mechanosensitive Ca(2+) channel blocker), while only RR inhibited Ca(2+) influx in SFBs. Our results su...Continue Reading

References

Jul 1, 1989·The Journal of Cell Biology·V P ShirinskyV N Smirnov
Dec 1, 1989·The Journal of Physiology·J R Levick, J N McDonald
Mar 7, 1998·The Biochemical Journal·P D'andreaM Grandolfo
Feb 15, 2001·Arthritis Research·G SchettJ Smolen
May 23, 2002·Biophysical Journal·Guillaume T Charras, Mike A Horton
Oct 18, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C Neidlinger-WilkeR Brand
Jun 11, 2003·The Journal of Cell Biology·Yuko IwataMunekazu Shigekawa
Jan 25, 2005·Nature Cell Biology·Rosario MarotoOwen P Hamill
Jun 1, 2005·Journal of Cell Science·Daniel BeckerMarina Jendrach
Mar 29, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·T S MombergerR M Mason
Sep 1, 2006·American Journal of Physiology. Cell Physiology·Tomohiro NumataYasunobu Okada
Nov 14, 2006·Biochemical and Biophysical Research Communications·Sachie OgataKanemitsu Shirasuna
Apr 11, 2007·Journal of Pharmacological Sciences·Hiromitsu MoritaJoseph E Brayden

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Citations

Aug 14, 2012·Archives of Dermatological Research·Chenyu HuangRei Ogawa
Aug 12, 2014·Biochemical and Biophysical Research Communications·Vladislav I Chubinskiy-NadezhdinElena A Morachevskaya

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