PMID: 11345434May 10, 2001Paper

Distinct modes of mature and precursor tRNA binding to Escherichia coli RNase P RNA revealed by NAIM analyses

RNA
C HeideR K Hartmann

Abstract

We have analyzed by nucleotide analog interference mapping (NAIM) pools of precursor or mature tRNA molecules, carrying a low level of Rp-RMPalphaS (R = A, G, I) or Rp-c7-deaza-RMPalphaS (R = A, G) modifications, to identify functional groups that contribute to the specific interaction with and processing efficiency by Escherichia coli RNase P RNA. The majority of interferences were found in the acceptor stem, T arm, and D arm, including the strongest effects observed at positions G19, G53, A58, and G71. In some cases (interferences at G5, G18, and G71), the affected functional groups are candidates for direct contacts with RNase P RNA. Several modifications disrupt intramolecular tertiary contacts known to stabilize the authentic tRNA fold. Such indirect interference effects were informative as well, because they allowed us to compare the structural constraints required for ptRNA processing versus product binding. Our ptRNA processing and mature tRNA binding NAIM analyses revealed overlapping but nonidentical patterns of interference effects, suggesting that substrate binding and cleavage involves binding modes or conformational states distinct from the binding mode of mature tRNA, the product of the reaction.

References

Jan 1, 1976·Annual Review of Biochemistry·A Rich, U L RajBhandary
Apr 15, 1977·Journal of Molecular Biology·A JackA Klug
Nov 19, 1976·Science·G J Quigley, A Rich
Dec 5, 1992·Journal of Molecular Biology·E L Christian, M Yarus
Jul 5, 1985·Journal of Molecular Biology·E WesthofD Moras
Oct 11, 1994·Nucleic Acids Research·B K Oh, N R Pace
Jul 25, 1993·Nucleic Acids Research·W D HardtR K Hartmann
May 25, 1993·Biochemistry·D Smith, N R Pace
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·T PanK Zhong
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·J Kufel, L A Kirsebom
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·J M WarneckeR K Hartmann
Oct 6, 1998·Annual Review of Biochemistry·D N Frank, N R Pace
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·J A PannucciJ W Brown

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Citations

Nov 15, 2002·Methods : a Companion to Methods in Enzymology·Eric L ChristianMichael E Harris
Sep 19, 2006·Nature Reviews. Microbiology·Alexei V Kazantsev, Norman R Pace
Nov 29, 2007·RNA·Miyun Kwon, Scott A Strobel
Apr 28, 2006·Journal of Molecular Biology·Ema KikovskaLeif A Kirsebom

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