PMID: 9166719Jun 1, 1997Paper

Distinct patterns of expression and physiological effects of sst1 and sst2 receptor subtypes in mouse hypothalamic neurons and astrocytes in culture

Journal of Neurochemistry
C ViolletJacques Epelbaum

Abstract

Somatostatin (SRIF) receptor subtypes (sst) were characterized in hypothalamic neurons and astrocytes by quantitative reverse transcription-polymerase chain reaction and radioreceptor assays using [125I-Tyr0,D-Trp8]SRIF-14 as a ligand in ionic conditions discriminating between SRIF-1 (sst2, -3, and -5 receptors) and SRIF-2 (sst1 and -4 receptors) binding sites. In neurons, sstl mRNA levels were twofold higher than those of sst2, and sst3-5 expression was only minor. Astrocytes expressed 10-fold less sst mRNAs than neurons, which corresponded mostly (80%) to sst2. SRIF-1 binding site radioautography indicated that 10% of hypothalamic neurons were labelled on both cell bodies and neuritic processes, as were 35% of astrocytes. On neuronal and glial membranes, SRIF-14 and octreotide, an sst2/sst3/sst5-selective analogue, completely displaced SRIF-1 binding, whereas des-AA(1,2,5)[D-Trp8,IAmp9]SRIF (CH-275), an sst1-selective analogue, was ineffective. Using SRIF-2 conditions, only SRIF-14 and CH-275 displaced the binding on neurons. No SRIF-2 binding was observed on glia. SRIF-14 and octreotide inhibited forskolin-stimulated adenylyl cyclase activity in neurons and glia, whereas CH-275 was effective in neurons only. In patch-clamp e...Continue Reading

Citations

Jun 6, 2002·Journal of Neuroendocrinology·P CerveraJ Epelbaum
Feb 4, 2003·Journal of Neurochemistry·Anna VasilakiKyriaki Thermos
Apr 14, 2016·Scientific Reports·Guillaume OsterstockPierre-François Méry
Jan 20, 2018·Physiological Reviews·Alexei Verkhratsky, Maiken Nedergaard
May 24, 2019·International Journal of Molecular Sciences·Maurizio CammalleriAlbertino Bigiani
Nov 23, 2000·Journal of Physiology, Paris·J Held-FeindtR Mentlein
Dec 1, 1999·Neuroscience·J JohnsonN C Brecha
Jan 16, 1999·Brain Research. Molecular Brain Research·J Held-FeindtR Mentlein
Jun 1, 2000·Regulatory Peptides·I SelmerP C Emson
Sep 26, 2003·Biochimica Et Biophysica Acta·Lars Neisig MøllerJens Juul Holst
Nov 9, 2005·Pharmacology & Therapeutics·Kyriaki ThermosDaniel Hoyer

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