PMID: 7527831Dec 1, 1994Paper

Distinct patterns of nitric oxide production in hepatic macrophages and endothelial cells following acute exposure of rats to endotoxin

Journal of Leukocyte Biology
D L LaskinJ D Laskin

Abstract

Hepatic macrophages and endothelial cells play an important role in the clearance of endotoxin from the portal circulation. These cells are activated by endotoxin to release reactive mediators including superoxide anion, hydrogen peroxide, and nitric oxide, which have been implicated in hepatic inflammation and tissue injury. In the present studies we analyzed mechanisms regulating the production of nitric oxide by hepatic macrophages and endothelial cells following in vivo exposure to endotoxin. Rats were injected intravenously with Escherichia coli lipopolysaccharide (LPS, 5 mg/kg). Cells were isolated from the animals 48 h later by in situ perfusion of the liver with collagenase and pronase followed by differential centrifugation and centrifugal elutriation. We found that macrophages and endothelial cells from both untreated and endotoxin-treated rats readily synthesized nitric oxide following in vitro stimulation with interferon-gamma (IFN-gamma) and LPS alone and in combination. This response was dependent on l-arginine and was blocked by two nitric oxide synthase inhibitors, NG-monomethyl-l-arginine and l-canavanine. Macrophages produced more nitric oxide in response to LPS or LPS plus IFN-gamma than endothelial cells. In...Continue Reading

Citations

Jan 1, 1996·Toxicologic Pathology·D L Laskin
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·E P NadlerH R Ford
Oct 12, 2007·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Naoyuki Matsuda, Yuichi Hattori
Jul 15, 2003·Clinical Microbiology Reviews·Edwin S Van AmersfoortJohan Kuiper
Jan 13, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D C RockeyP W Noble
Jan 13, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V ShahR J Groszmann
Jun 9, 2001·Antioxidants & Redox Signaling·J D LaskinD L Laskin
Jan 11, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amy N MacRitchieDiana M Stafforini
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N AhmadD L Laskin
Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C R GardnerD L Laskin
May 9, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Douglas D Bannerman, Simeon E Goldblum
Oct 26, 2007·Anesthesia and Analgesia·E Gina Votta-VelisIrina V Balyasnikova
Sep 13, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Aymn T AbbasDiaa T A Youssef
Apr 10, 2018·Chemico-biological Interactions·Taskeen Fathima DocratAnil A Chuturgoon
Feb 9, 2002·Journal of Biochemical and Molecular Toxicology·K AlamA M Al-Bekairi
Jul 8, 1999·European Journal of Pharmacology·S CuzzocreaA P Caputi
Mar 10, 2010·Free Radical Biology & Medicine·Syed Mohsin WaheedDennis J Stuehr

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