Distinct Populations in Spin-Label EPR Spectra from Nitroxides

The Journal of Physical Chemistry. B
D Marsh

Abstract

Two-component nitroxide spin-label electron paramagnetic presonance (EPR) spectra are important to the analysis of lipid-protein interactions, phase separation in lipid membranes, and conformational changes in proteins. A paper published in this journal offers an interpretation of such spectra based on simulations with single-site models. It is not possible to reproduce those simulations published in Meirovitch , E. ; [ Analysis of Protein Lipid Interactions Based on Model Simulations of Electron Spin Resonance Spectra . J. Phys. Chem. 1984 , 88 , 3454 - 3465 ] that might conceivably be taken to resemble two-component line shapes, when using the motional model and parameters given in that paper. Instead of the apparent two components, the spectra resemble single-component powder patterns expected from axially anisotropic, partial motional-averaging (a situation familiar for chain-labeled lipids in nonaligned fluid membranes). This is because: (i) the nitroxide z-axis is inclined at a fixed angle to the principal diffusion axis, and (ii) motion perpendicular to the principal diffusion axis is so slow as to approximate a powder distribution. The line shapes are compatible with simulations that use the same model for a complete ra...Continue Reading

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Marsh, F J Barrantes
Jan 18, 1973·The Journal of Physical Chemistry·E L Wee, W G Miller
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·P C JostG Vanderkooi
Jan 27, 1971·Journal of the American Chemical Society·W L Hubbell, H M McConnell
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J R BrotherusL E Hokin
Sep 1, 1981·Biochemistry·J R BrotherusL E Hokin
Jan 27, 2005·Biochimica Et Biophysica Acta·Yun-Wei ChiangGerald W Feigenson
Sep 29, 2005·Journal of Magnetic Resonance·Stefan Stoll, Arthur Schweiger
Nov 18, 2010·Biophysical Journal·Frederick A HeberleGerald W Feigenson
Jan 26, 2018·Applied Magnetic Resonance·Derek Marsh

❮ Previous
Next ❯

Citations

Jun 30, 2021·Biochimica Et Biophysica Acta. Biomembranes·Matthias HoffmannDariush Hinderberger
Jul 30, 2021·Biomacromolecules·Matthias HoffmannDariush Hinderberger
Feb 16, 2019·The Journal of Physical Chemistry. B·Eva MeirovitchJack H Freed
Feb 16, 2019·The Journal of Physical Chemistry. B·Derek Marsh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.