Distinct properties of the five UDP-D-glucose/UDP-D-galactose 4-epimerase isoforms of Arabidopsis thaliana.

The Journal of Biological Chemistry
Christine BarberGeorg J Seifert

Abstract

Plant genomes contain genetically encoded isoforms of most nucleotide sugar interconversion enzymes. Here we show that Arabidopsis thaliana has five genes encoding functional UDP-D-glucose/UDP-D-galactose 4-epimerase (named UGE1 to UGE5). All A. thaliana UDP-d-glucose 4-epimerase isoforms are dimeric in solution, maximally active in vitro at 30-40 degrees C, and show good activity between pH 7 and pH 9. In vitro, UGE1, -3, and -5 act independently of externally added NAD+, whereas cofactor addition stimulates the activity of UGE2 and is particularly important for UGE4 activity. UGE1 and UGE3 are most efficiently inhibited by UDP. The five isoforms display kcatUDP-Gal values between 23 and 128 s(-1) and KmUDP-Gal values between 0.1 and 0.3 mm. This results in enzymatic efficiencies ranging between 97 and 890 mm(-1) s(-1) for UGE4 = UGE1 < UGE3 < UGE5 < UGE2. The KmUDP-Glc values, derived from the Haldane relationship, were 0.76 mm for UGE1, 0.56 mm for UGE4, and between 0.13 and 0.23 mm for UGE2, -3, and -5. The expression of UGE isoforms is ubiquitous and displays developmental and cell type-dependent variations. UGE1 and -3 expression patterns globally resemble enzymes involved in carbohydrate catabolism, and UGE2, -4, and -5 ...Continue Reading

References

Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·R A Darrow, R Rodstrom
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·P Dörmann, C Benning
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·M A BlázquezC Gancedo
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Sep 26, 2000·Molecular Genetics and Metabolism·G Novelli, J K Reichardt
Apr 15, 2003·Plant Physiology·Joost T van DongenPeter Geigenberger
Aug 19, 2003·The Journal of Biological Chemistry·Hazel M HoldenJames B Thoden
Dec 13, 2003·Science·Kenneth BirnbaumPhilip N Benfey
Feb 20, 2004·The Plant Cell·Georg J SeifertKeith Roberts
Jul 13, 2004·Bioinformatics·Dirk SteinhauserJoachim Kopka
Sep 3, 2004·The Plant Journal : for Cell and Molecular Biology·Nicholas A JolliffeLorenzo Frigerio
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Oct 7, 2004·Molecular Genetics and Metabolism·Kerry L RossJudith L Fridovich-Keil
Feb 24, 2005·Annual Review of Plant Biology·Brenda S J Winkel
Mar 30, 2005·The Journal of Biological Chemistry·James B Thoden, Hazel M Holden

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Citations

Jul 1, 2009·Journal of Biochemistry·Xiaogang GuMaor Bar-Peled
Sep 30, 2008·Eukaryotic Cell·Frédérique MoyrandGuilhem Janbon
Mar 5, 2011·Annual Review of Plant Biology·Maor Bar-Peled, Malcolm A O'Neill
Mar 5, 2013·Annual Review of Plant Biology·Melani A AtmodjoDebra Mohnen
Oct 7, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Saren Gaowa, Shicui Zhang
Oct 13, 2015·Journal of Proteomics·Puneet PaulEnrico Schleiff
Sep 4, 2007·The Plant Journal : for Cell and Molecular Biology·Sandra C Sharples, Stephen C Fry
Oct 3, 2006·Current Opinion in Plant Biology·Olivier LerouxelKenneth Keegstra
Oct 9, 2014·Frontiers in Plant Science·Maria C AriasChristophe D'Hulst
Mar 2, 2016·Annual Review of Plant Biology·Markus Pauly, Kenneth Keegstra
Nov 3, 2009·Progress in Lipid Research·Jacques JoyardNorbert Rolland
Jan 1, 2020·Journal of Asian Natural Products Research·Yan YangWei Wang
Oct 6, 2020·Frontiers in Plant Science·Marina CianciaRando Tuvikene
Jun 22, 2017·Journal of Experimental Botany·Joakim BygdellGunnar Wingsle
Feb 23, 2021·Journal of Integrative Plant Biology·Yuanyan ZhangJianmin Wan
Sep 6, 2018·Biochemical and Biophysical Research Communications·Chul-Hyun AhnPhun Bum Park
Jun 16, 2021·Plant & Cell Physiology·Alban MarietteEdwin R Lampugnani

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