Distinct RAD51 associations with RAD52 and BCCIP in response to DNA damage and replication stress.

Cancer Research
Justin WrayZhiyuan Shen

Abstract

RAD51 has critical roles in homologous recombination (HR) repair of DNA double-strand breaks (DSB) and restarting stalled or collapsed replication forks. In yeast, Rad51 function is facilitated by Rad52 and other "mediators." Mammalian cells express RAD52, but BRCA2 may have supplanted RAD52 in mediating RAD51 loading onto ssDNA. BCCIP interacts with BRCA2, and both proteins are important for RAD51 focus formation after ionizing radiation and HR repair of DSBs. Nonetheless, mammalian RAD52 shares biochemical activities with yeast Rad52, including RAD51 binding and single-strand annealing, suggesting a conserved role in HR. Because RAD52 and RAD51 associate, and RAD51 and BCCIP associate, we investigated the colocalization of RAD51 with BCCIP and RAD52 in human cells. We found that RAD51 colocalizes with BCCIP early after ionizing radiation, with RAD52 later, and there was little colocalization of BCCIP and RAD52. RAD52 foci are induced to a greater extent by hydroxyurea, which stalls replication forks, than by ionizing radiation. Using fluorescence recovery after photo bleaching, we show that RAD52 mobility is reduced to a greater extent by hydroxyurea than ionizing radiation. However, BCCIP showed no changes in mobility after ...Continue Reading

References

Jan 5, 1996·The Journal of Biological Chemistry·Z ShenM S Park
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Tsuzuki MoritaT
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·U H MortensenR Rothstein
Mar 21, 2001·EMBO Reports·Y Liu, N Maizels
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M LisbyU H Mortensen
Nov 29, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lorraine S Symington
Feb 28, 2003·Oncogene·Madalena TarsounasStephen C West
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·J F Ward
May 27, 2003·Nature Cell Biology·Michael LisbyRodney Rothstein
Aug 5, 2003·Molecular and Cellular Biology·Kai RothkammMarkus Löbrich
Aug 5, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Duane R PilchWilliam M Bonner
Aug 13, 2003·The Journal of Biological Chemistry·Patrick SungMichael G Sehorn
Aug 30, 2003·Oncogene·Kristoffer Valerie, Lawrence F Povirk
Dec 19, 2003·Genes & Development·Mikhail LomonosovAshok R Venkitaraman
May 18, 2004·Current Opinion in Cell Biology·Michael Lisby, Rodney Rothstein
Jun 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jaspal K Kumar, Ravindra C Gupta
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Jiri BartekJiri Lukas
Feb 24, 2006·Carcinogenesis·Paul R AndreassenAlan D D'Andrea
Feb 1, 2007·DNA and Cell Biology·Emil MladenovBoyka Anachkova

❮ Previous
Next ❯

Citations

Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Zhihui FengSimon N Powell
Feb 1, 2011·Journal of Molecular Cell Biology·Chris AllenJac A Nickoloff
Apr 3, 2012·Nucleic Acids Research·Lumir KrejciXiaolan Zhao
Aug 6, 2009·BMC Cancer·Jingmei LiuZhiyuan Shen
Nov 10, 2013·PLoS Genetics·Ivana MurfuniPietro Pichierri
Jan 10, 2012·Biochemical Pharmacology·Zhengguan YangMichael D Wyatt
Nov 10, 2015·Chemistry & Biology·Gurushankar ChandramoulyRichard T Pomerantz
Oct 8, 2014·DNA Repair·Ann C ParplysClaudia Wiese
Dec 17, 2016·Molecular Cell·Sotirios K SotiriouThanos D Halazonetis
Aug 18, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Brian T WilsonTuomo Polvikoski
Nov 18, 2017·Breast Cancer Research : BCR·Robert HromasKimi Y Kong
Feb 14, 2020·Cancers·Eva MalacariaPietro Pichierri
Feb 13, 2020·Cancers·Eric A Hendrickson
Jan 1, 2012·Biomolecules·Audrey Costes, Sarah A E Lambert
May 12, 2010·Nucleic Acids Research·Leyma P De HaroJac A Nickoloff
Oct 19, 2017·Nature Communications·Sofija MijicMassimo Lopes
Oct 20, 2017·Breast Cancer Research : BCR·Roberto Droz-RosarioZhiyuan Shen
Oct 18, 2013·Cell Cycle·Zhiyuan Shen, Steven Huhn
Mar 31, 2019·Nature Communications·Eva MalacariaPietro Pichierri
Jul 8, 2020·Seminars in Cell & Developmental Biology·Alexander Carver, Xiaodong Zhang
Oct 12, 2020·The American Journal of Pathology·Huimei LuZhiyuan Shen
Feb 1, 2020·Pharmacology & Therapeutics·Erik LauriniSabrina Pricl
Jul 21, 2021·International Journal of Radiation Biology·Jac A NickoloffRobert Hromas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.