Distinct regulatory effects of the Na,K-ATPase gamma subunit

The Journal of Biological Chemistry
Helen X PuRhoda Blostein

Abstract

The two variants of the gamma subunit of the rat renal sodium pump, gamma(a) and gamma(b), have similar effects on the Na,K-ATPase. Both increase the affinity for ATP due to a shift in the enzyme's E(1) <--> E(2) conformational equilibrium toward E(1). In addition, both increase K(+) antagonism of cytoplasmic Na(+) activation. To gain insight into the structural basis for these distinct effects, extramembranous N-terminal and C-terminal mutants of gamma were expressed in rat alpha1-transfected HeLa cells. At the N terminus, the variant-distinct region was deleted (gammaNDelta7) or replaced by alanine residues (gammaN7A). At the C terminus, four (gamma(a)CDelta4) or ten (gamma(a)CDelta10) residues were deleted. None of these mutations abrogates the K(+)/Na(+) antagonism as evidenced in a similar increase in K'(Na) seen at high (100 mm) K(+) concentration. In contrast, the C-terminal as well as N-terminal deletions (gammaNDelta7, gamma(a)CDelta4, and gamma(a)CDelta10) abolished the decrease in K'(ATP) seen with wild-type gamma(a) or gamma(b). It is concluded that different regions of the gamma chain mediate the distinct functional effects of gamma, and the effects can be long-range. In the transmembrane region, the impact of G41R...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R GoldshlegerS J Karlish
Jan 1, 1990·Genomics·B A OostraB Wieringa
Aug 1, 1990·The American Journal of Physiology·C Barlet-BasA Doucet
Jun 1, 1988·The Journal of Physiology·J R Sachs
Jun 1, 1973·The Journal of Physiology·R P Garay, P J Garrahan
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·B AttaliH Garty
Sep 1, 1996·The American Journal of Physiology·C CapurroN Farman
Jan 24, 1998·The Journal of Biological Chemistry·A G TherienR Blostein
Apr 23, 1999·The Journal of Biological Chemistry·A G TherienR Blostein
Nov 24, 1999·The Journal of Biological Chemistry·E ArystarkhovaK J Sweadner
Aug 16, 2000·American Journal of Physiology. Cell Physiology·A G Therien, R Blostein
Dec 9, 2000·Biochemical and Biophysical Research Communications·K J SweadnerE Arystarkhova
Feb 22, 2001·American Journal of Physiology. Renal Physiology·H ShiH Garty
Aug 15, 2001·American Journal of Physiology. Renal Physiology·R K Wetzel, K J Sweadner
Jan 5, 2002·Journal of Bioenergetics and Biomembranes·A G TherienR Blostein

❮ Previous
Next ❯

Citations

Jan 23, 2003·Biopolymers·Anthony W PartridgeCharles M Deber
Jul 12, 2005·The Journal of Membrane Biology·A ZouzoulasR Blostein
Jan 14, 2011·The Journal of Biological Chemistry·Neeraj Kumar MishraSteven J D Karlish
Apr 5, 2011·The Journal of Biological Chemistry·Kathleen J SweadnerElena Arystarkhova
Feb 8, 2006·Annual Review of Physiology·Haim Garty, Steven J D Karlish
May 2, 2008·American Journal of Physiology. Renal Physiology·Qun ShaRobert W Mercer
Apr 6, 2007·Biological & Pharmaceutical Bulletin·Junko ArimochiMasatomo Maeda
Jun 19, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Linda W Chang, Nicholas C Spitzer
Jul 11, 2006·Médecine sciences : M/S·Benjamin DelpratKäthi Geering
May 24, 2003·Annals of the New York Academy of Sciences·Rhoda BlosteinAthina Zouzoulas
May 16, 2013·Journal of Molecular and Cellular Cardiology·Davor PavlovicMichael J Shattock
Jan 23, 2003·Science's STKE : Signal Transduction Knowledge Environment·Gilles Crambert, Käthi Geering
Aug 9, 2003·The Journal of Biological Chemistry·Athina ZouzoulasRhoda Blostein
Jul 6, 2004·The Journal of Biological Chemistry·Ciming LiKäthi Geering
Nov 26, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Li-Guo JiaAmy L Tucker
Mar 10, 2005·The Journal of Biological Chemistry·D Holstead JonesGerald M Kidder
May 24, 2003·Annals of the New York Academy of Sciences·Iwan C MeijNine V A M Knoers
May 24, 2003·Annals of the New York Academy of Sciences·Käthi GeeringGilles Crambert
May 24, 2003·Annals of the New York Academy of Sciences·Kaarina Pihakaski-MaunsbachArvid B Maunsbach
May 24, 2003·Annals of the New York Academy of Sciences·Kathleen J SweadnerRandall K Wetzel
Jun 20, 2008·Journal of the American Society of Nephrology : JASN·R Todd AlexanderRené J Bindels
Jan 13, 2006·American Journal of Physiology. Renal Physiology·Käthi Geering
Feb 1, 2006·The Journal of Biological Chemistry·Athina Zouzoulas, Rhoda Blostein
Oct 20, 2006·American Journal of Physiology. Cell Physiology·Elena ArystarkhovaKathleen J Sweadner
May 17, 2003·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Flemming Cornelius, Yasser A Mahmmoud
Mar 6, 2004·American Journal of Physiology. Renal Physiology·Martin KonradThomas Gudermann
Mar 27, 2003·American Journal of Physiology. Renal Physiology·Randall K Wetzel, Kathleen J Sweadner
Sep 4, 2018·Physiological Reports·Haim MayanSteven J D Karlish
Dec 24, 2005·The Journal of Biological Chemistry·Moshit LindzenSteven J D Karlish
Mar 11, 2021·The Journal of General Physiology·John Q YapSeth L Robia
Sep 28, 2010·Archives of Biochemistry and Biophysics·Vanessa Faria CortesCarlos Frederico Leite Fontes
May 17, 2012·Biochimica Et Biophysica Acta·Elias C C SilvaCarlos Frederico L Fontes
Dec 31, 2002·Journal of the American Society of Nephrology : JASN·Martin Konrad, Stefanie Weber
Sep 6, 2005·Seminars in Nephrology·Haim Garty, Steven J D Karlish

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.