Distinct requirements for chromatin assembly in transcriptional repression by thyroid hormone receptor and histone deacetylase

The EMBO Journal
J WongA P Wolffe

Abstract

Histone deacetylase and chromatin assembly contribute to the control of transcription of the Xenopus TRbetaA gene promoter by the heterodimer of Xenopus thyroid hormone receptor and 9-cis retinoic acid receptor (TR-RXR). Addition of the histone deacetylase inhibitor Trichostatin A (TSA) relieves repression of transcription due to chromatin assembly following microinjection of templates into Xenopus oocyte nuclei, and eliminates regulation of transcription by TR-RXR. Expression of Xenopus RPD3p, the catalytic subunit of histone deacetylase, represses the TRbetaA promoter, but only after efficient assembly of the template into nucleosomes. In contrast, the unliganded TR-RXR represses templates only partially assembled into nucleosomes; addition of TSA also relieves this transcriptional repression. This result indicates the distinct requirements for chromatin assembly in mediating transcriptional repression by the deacetylase alone, compared with those needed in the presence of unliganded TR-RXR. In addition, whereas hormone-bound TR-RXR targets chromatin disruption as assayed through changes in minichromosome topology and loss of a regular nucleosomal ladder on micrococcal nuclease digestion, addition of TSA relieves transcriptio...Continue Reading

References

May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J E GermondP Chambon
Oct 10, 1975·Science·A Ruiz-CarrilloV G Allfrey
Oct 11, 1990·Nucleic Acids Research·G AlmouzniA P Wolffe
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y YaoitaD D Brown
Jan 1, 1981·Annual Review of Genetics·J B Gurdon, D A Melton
Jun 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P R Cook
Aug 1, 1995·Genes & Development·B P Leblanc, H G Stunnenberg
May 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M YoshidaT Beppu
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·R E SobelC D Allis
Aug 11, 1994·Nature·A N ImbalzanoR E Kingston
Feb 25, 1994·Journal of Molecular Biology·W R BauerA P Wolffe
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A BaniahmadB W O'Malley
Dec 15, 1995·Cell·D J Mangelsdorf, R M Evans
Dec 15, 1995·Genes & Development·A AlevizopoulosN Mermod

❮ Previous
Next ❯

Citations

Jan 1, 1997·Biopolymers·A Travers, H Drew
Nov 7, 1998·Molecular and Cellular Endocrinology·G Jenster
Jun 30, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·L M SachsA Ishizuya-Oka
Jun 26, 1998·Current Opinion in Cell Biology·P D Varga-Weisz, P B Becker
Oct 6, 1999·Current Opinion in Genetics & Development·B D Lemon, L P Freedman
Jul 6, 2000·Trends in Endocrinology and Metabolism : TEM·Y Wu, R J Koenig
May 14, 1999·Trends in Endocrinology and Metabolism : TEM·Q LiA P Wolffe
Nov 5, 1999·Trends in Endocrinology and Metabolism : TEM·L P Freedman
Jun 13, 2008·Journal de la Société de biologie·Emmanuelle HavisLaurent M Sachs
May 23, 2002·DNA and Cell Biology·Stéphane Veilleux, Guylain Boissonneault
Aug 15, 2009·Thyroid : Official Journal of the American Thyroid Association·Yun-Bo Shi
Mar 4, 2000·The EMBO Journal·S BelikovO Wrange
Jan 16, 1999·Nucleic Acids Research·A P Wolffe, J J Hayes
Apr 25, 2007·Molecular and Cellular Biology·Junjiang FuJiemin Wong
Sep 1, 2006·Molecular and Cellular Biology·M David StewartJiemin Wong
Nov 13, 2003·Molecular and Cellular Biology·Simona SegallaNicoletta Landsberger
Nov 30, 2005·Molecular and Cellular Biology·Natalia P Ulyanova, Gavin R Schnitzler
Jun 9, 2000·Annual Review of Physiology·J Zhang, M A Lazar
Oct 27, 2007·BMC Genomics·Corinne BressonSandrine Gonin-Giraud
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·A A CarmenM Grunstein
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·L M Sachs, Y B Shi
Mar 31, 2005·Current Opinion in Genetics & Development·Raphael MargueronDanny Reinberg
Mar 24, 1999·Trends in Biochemical Sciences·A Travers
Oct 23, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L M SachsY B Shi
Jul 30, 2015·Development, Growth & Differentiation·Luan Wen, Yun-Bo Shi
Sep 30, 2010·Virus Research·Matthew B Reeves
Jul 24, 2004·Differentiation; Research in Biological Diversity·Stephanie M M Cossette, Thomas A Drysdale
May 9, 2012·Biochimica Et Biophysica Acta·Alexis GrimaldiLaurent M Sachs
May 12, 2000·Journal of Structural Biology·A P Wolffe, D Guschin

❮ Previous
Next ❯

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