Distinct roles of Mus81, Yen1, Slx1-Slx4, and Rad1 nucleases in the repair of replication-born double-strand breaks by sister chromatid exchange.

Molecular and Cellular Biology
Sandra Muñoz-GalvánAndrés Aguilera

Abstract

Most spontaneous DNA double-strand breaks (DSBs) arise during replication and are repaired by homologous recombination (HR) with the sister chromatid. Many proteins participate in HR, but it is often difficult to determine their in vivo functions due to the existence of alternative pathways. Here we take advantage of an in vivo assay to assess repair of a specific replication-born DSB by sister chromatid recombination (SCR). We analyzed the functional relevance of four structure-selective endonucleases (SSEs), Yen1, Mus81-Mms4, Slx1-Slx4, and Rad1, on SCR in Saccharomyces cerevisiae. Physical and genetic analyses showed that ablation of any of these SSEs leads to a specific SCR decrease that is not observed in general HR. Our work suggests that Yen1, Mus81-Mms4, Slx4, and Rad1, but not Slx1, function independently in the cleavage of intercrossed DNA structures to reconstitute broken replication forks via HR with the sister chromatid. These unique effects, which have not been detected in other studies unless double mutant combinations were used, indicate the formation of distinct alternatives for the repair of replication-born DSBs that require specific SSEs.

References

Oct 6, 1994·Nature·Y HabrakenS Prakash
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·F Pâques, J E Haber
Oct 5, 2001·Molecular and Cellular Biology·R K ChakravertyI D Hickson
Oct 20, 2001·Genes & Development·V KaliramanS J Brill
Jun 27, 2002·The Journal of Biological Chemistry·Claudette L DoeMatthew C Whitby
Dec 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Francis FabreSerge Gangloff
May 2, 2003·Molecular and Cellular Biology·Suzanne A Bastin-ShanowerSteven J Brill
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Stephen C West
Jun 25, 2003·Molecular Cell·Sergio González-BarreraAndrés Aguilera
Jul 2, 2003·Genes & Development·William M Fricke, Steven J Brill
Oct 7, 2003·Molecular Biology of the Cell·Stéphane CoulonPaul Russell
Dec 14, 2004·DNA Repair·William M FrickeSteven J Brill
Jul 29, 2006·Cell·Emmanuelle MartiniScott Keeney
Aug 8, 2006·Nature Cell Biology·Giacomo De PiccoliLuis Aragón
Oct 16, 2007·Nature Structural & Molecular Biology·Katsuhiro HanadaRoland Kanaar
Jan 30, 2008·Nature Reviews. Genetics·Andrés Aguilera, Belén Gómez-González
Feb 19, 2008·Nucleic Acids Research·Kirk Tevebaugh Ehmsen, Wolf-Dietrich Heyer
Mar 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ewan R Taylor, Clare H McGowan
Apr 5, 2008·The EMBO Journal·Laura RoseaulinBenoit Arcangioli
Jun 4, 2008·Annual Review of Biochemistry·Alberto CicciaStephen C West
Nov 21, 2008·Nature·Stephen C Y IpStephen C West
Jan 21, 2009·Cellular and Molecular Life Sciences : CMLS·B PardoA Aguilera
Feb 25, 2010·The Journal of Biological Chemistry·Ye Dee Tay, Leonard Wu
Jul 17, 2010·Genes & Development·Ulrich RassStephen C West
Aug 10, 2010·Annual Review of Genetics·Wolf-Dietrich HeyerJie Liu

❮ Previous
Next ❯

Citations

Aug 14, 2012·Nature Structural & Molecular Biology·Gerard MazónLorraine S Symington
Apr 16, 2013·Nature Structural & Molecular Biology·Pierre-Marie DehéPierre-Henri L Gaillard
Aug 1, 2013·Nucleic Acids Research·Irene SaugarJosé Antonio Tercero
May 31, 2012·Molecular and Cellular Biology·Erin K SchwartzWolf-Dietrich Heyer
Oct 2, 2013·BioMed Research International·Annapaola Franchitto
Nov 10, 2013·PLoS Genetics·Ivana MurfuniPietro Pichierri
Nov 14, 2012·Annual Review of Genetics·Anna Malkova, James E Haber
Oct 22, 2013·Genes & Development·Ryan L RaglandEric J Brown
Jun 15, 2014·FEBS Letters·Zdenka Bartosova, Lumir Krejci
Oct 23, 2015·Nucleic Acids Research·Diego DibitettoAchille Pellicioli
Jun 26, 2015·FEMS Microbiology Reviews·Adrianna SkonecznaMarek Skoneczny
Mar 15, 2015·Nucleic Acids Research·Melita ChavdarovaLumir Krejci
Oct 15, 2013·Molecular Cell·Gerard Mazón, Lorraine S Symington
Aug 12, 2016·FEMS Yeast Research·Ibtissam TalhaouiGerard Mazón
Apr 22, 2017·Critical Reviews in Biochemistry and Molecular Biology·Cynthia J Sakofsky, Anna Malkova
Jun 15, 2013·Biomolecules·Cynthia J SakofskyAnna Malkova
Nov 28, 2017·Annual Review of Genetics·Philippe Pasero, Alessandro Vindigni
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Andrew H LeeDavid A Fidock
Dec 19, 2018·Genes·Seong Min Kim, Susan L Forsburg
Jul 8, 2017·Genetics·Christopher D Putnam, Richard D Kolodner
Nov 28, 2018·Nature Communications·Ibtissam TalhaouiGerard Mazón
Oct 4, 2017·BMC Biology·Alexandra SisakovaLumir Krejci
Feb 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Romulo SegoviaPeter C Stirling
Oct 20, 2020·PLoS Biology·Nathan PalmerPhilipp Kaldis
Nov 12, 2019·Molecular Cell·Dominick A MatosLee Zou
Jul 20, 2014·Plant Physiology·Markus Bauknecht, Daniela Kobbe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.