Distinct roles of P(II)-like signal transmitter proteins and amtB in regulation of nif gene expression, nitrogenase activity, and posttranslational modification of NifH in Azoarcus sp. strain BH72.

Journal of Bacteriology
Dietmar E Martin, Barbara Reinhold-Hurek

Abstract

P(II)-like signal transmitter proteins, found in Bacteria, Archaea, and plants, are known to mediate control of carbon and nitrogen assimilation. They indirectly regulate the activity of key metabolic enzymes and transcription factors by protein-protein interactions with signal transduction proteins. Many Proteobacteria harbor two paralogous P(II)-like proteins, GlnB and GlnK, whereas a novel third P(II) paralogue (GlnY) was recently identified in Azoarcus sp. strain BH72, a diazotrophic endophyte of grasses. In the present study, evidence was obtained that the P(II)-like proteins have distinct roles in mediating nitrogen and oxygen control of nif gene transcription and nitrogenase activity. Full repression of nif gene transcription in the presence of a combined nitrogen source or high oxygen concentrations was observed in wild-type and glnB and glnK knockout mutants, revealing that GlnB and GlnK can complement each other in mediating the repression. In contrast, in a glnBK double mutant strain in the presence of only GlnY, nif gene transcription was still detectable, albeit at a lower level, on nitrate or 20% oxygen. As another level of control, nitrogenase activity was regulated by at least three types of mechanisms in strain...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·E R Stadtman
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 1, 1989·Journal of Bacteriology·H A FuR H Burris
Jan 1, 1989·Current Topics in Cellular Regulation·P W Ludden, G P Roberts
Apr 1, 1985·Journal of Bacteriology·B ReinholdI Fendrik
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M R PopeP W Ludden
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 27, 1995·The Journal of Biological Chemistry·Y Liu, M L Kahn
Apr 1, 1994·Journal of Bacteriology·T HurekE Kellenberger
Sep 6, 1993·FEBS Letters·S Colonna-RomanoR Defez
Feb 16, 1993·Biochemistry·D Cervantes-LaureanM K Jacobson
Dec 1, 1995·Microbiological Reviews·M J Merrick, R A Edwards
Jul 1, 1996·Molecular Microbiology·W C van HeeswijkH V Westerhoff
Jul 1, 1997·Journal of Bacteriology·E M BenelliF O Pedrosa
Nov 15, 1997·Applied and Environmental Microbiology·T HurekB Reinhold-Hurek
May 20, 1998·Trends in Microbiology·B Reinhold-Hurek, T Hurek
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·E SoupeneS Kustu
Jun 11, 1998·Journal of Bacteriology·D MeletzusC Kennedy
Oct 24, 1998·Molecular Microbiology·J DörrB Reinhold-Hurek
Aug 3, 1999·Genetics·P S Kessler, J A Leigh
Jan 19, 2000·Trends in Genetics : TIG·G ThomasM Merrick
Feb 7, 2001·Trends in Microbiology·A J Ninfa, M R Atkinson
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·W C van HeeswijkS G Vasudevan
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·T ArcondéguyM Merrick
Jul 11, 2002·Journal of Bacteriology·Alexander F Yakunin, Patrick C Hallenbeck
Sep 1, 1986·Applied and Environmental Microbiology·B ReinholdI Fendrik

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Citations

Dec 7, 2005·Archives of Microbiology·Lilian NoindorfLeda S Chubatsu
Dec 4, 2003·Journal of Biotechnology·Thomas Hurek, Barbara Reinhold-Hurek
Jan 18, 2011·Journal of Experimental Botany·Tatiana KraiserRodrigo A Gutiérrez
Sep 10, 2005·Applied and Environmental Microbiology·Giseli KlassenFábio O Pedrosa
Dec 19, 2008·Science in China. Series C, Life Sciences·Ying Zhang, JinDong Zhao
Sep 27, 2003·Environmental Microbiology·Zhiyuan TanBarbara Reinhold-Hurek
Aug 7, 2012·FEMS Microbiology Reviews·Luciano F HuergoMike Merrick
Oct 22, 2005·FEMS Microbiology Letters·Luciano F HuergoEmanuel M Souza
Dec 23, 2011·Molecular Microbiology·Abhijit SarkarBarbara Reinhold-Hurek

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