Distinct roles of transcription factors TFIIIB and TFIIIC in RNA polymerase III transcription reinitiation

Proceedings of the National Academy of Sciences of the United States of America
Roberto FerrariGiorgio Dieci

Abstract

Eukaryotic RNA polymerase (Pol) III is recruited to target promoters by a stable preinitiation complex containing transcription factors TFIIIC and TFIIIB. After the first transcription cycle, reinitiation proceeds through facilitated recycling, a process by which the terminating Pol III rapidly reloads onto the same transcription unit. Here, we show that Pol III is repeatedly recaptured in vitro by the first transcribed gene, even in the presence of a juxtaposed competitor promoter complex, thus suggesting that facilitated recycling is not merely due to a stochastic reassociation process favored by the small size of class III genes. The transcription factor requirements for facilitated reinitiation were investigated by taking advantage of Pol III templates that support both TFIIIC-dependent and TFIIIC-independent transcription. A TFIIIC-less transcription system, in which TFIIIB was reconstituted from recombinant TATA box-binding protein and Brf1 proteins and a crude fraction containing the Bdp1 component, was sufficient to direct efficient Pol III recycling on short ( approximately 100 bp) class III genes. Unexpectedly, however, on longer (>300 bp) transcription units, reinitiation in the presence of TFIIIB alone was compromis...Continue Reading

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Citations

Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Arounie TavenetJoël Acker
Aug 1, 2008·The Journal of Biological Chemistry·Elisabetta Soragni, George A Kassavetis
Nov 1, 2008·The Journal of Biological Chemistry·Pavel CabartIan M Willis
Mar 23, 2011·Nucleic Acids Research·Andrea OrioliGiorgio Dieci
Jun 3, 2009·Genes & Development·Patricia Richard, James L Manley
May 14, 2008·Molecular and Cellular Biology·Sarah L FrenchAnn L Beyer
Sep 17, 2005·Molecular and Cellular Biology·Christine ConesaGiorgio Dieci
Oct 9, 2012·Biochimica Et Biophysica Acta·Giorgio DieciDavide Carnevali
Oct 16, 2012·Biochimica Et Biophysica Acta·Joël AckerOlivier Lefebvre
Jun 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nazif AlicChristophe Carles
Nov 7, 2012·Biochimica Et Biophysica Acta·Giorgio DieciRoberto Ferrari
Oct 27, 2012·Biochimica Et Biophysica Acta·Aneeshkumar G ArimbasseriRichard J Maraia
Nov 21, 2012·Biochimica Et Biophysica Acta·Robyn D Moir, Ian M Willis
Oct 30, 2007·Cellular & Molecular Biology Letters·Roberto Ferrari, Giorgio Dieci
Mar 25, 2005·The Journal of Biological Chemistry·Priscilla BragliaGiorgio Dieci
Mar 7, 2006·The Journal of Biological Chemistry·Cécile DucrotJoel Acker
Jun 22, 2006·The Journal of Biological Chemistry·Elisa GuffantiGiorgio Dieci
Jan 22, 2011·Critical Reviews in Biochemistry and Molecular Biology·Sung-Hoon JunKatsuhiko S Murakami
Jan 1, 2014·Transcription·Giorgio DieciMaria Cristina Bosio
Sep 25, 2018·Nucleic Acids Research·Maria C HerreraJorrit M Enserink
Jan 25, 2020·Nature Communications·Timothy T HardenJeff Gelles
Mar 17, 2021·International Journal for Parasitology·H A Walters, L A Temesvari
Apr 4, 2021·Genes·Abigail K HuffinesDavid A Schneider
Aug 17, 2021·Frontiers in Molecular Biosciences·Elisabeth LataMartin Teichmann
Nov 5, 2021·Nature Communications·Zhong ChenQianben Wang

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